Technique for differential display

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, C12Q 168, C12P 1934

Patent

active

060459980

ABSTRACT:
The method of this invention relates to the use of improved anchor primers and a novel purification process to increase the efficiency and accuracy of the differential display technique.

REFERENCES:
patent: 5599672 (1997-02-01), Liang et al.
patent: 5665547 (1997-09-01), Pardee et al.
Liang and Pardee. Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction. Science 257: 967-971, Aug. 1992.
Liang et al. Analysis of Altered Gene Expression by Differential Display. Methods Enzymol. 254: 304-321, 1995.
Liang et al. Recent Advances in Differential Display. Curr. Opinion Immunol. 7: 274-280, 1995.
JPO Abstract No. JP406303997A, "Determination of cDNA", Takagi et al, Nov. 1, 1994.
Ace C.I., Balsamo M., Le L.T., Okulicz W.C. (1994) Isolation of Progesterone-Dependent Complementary Deoxyribonucleic Acid Fragments from Rhesus Monkey Endometrium by Sequential Subtractive Hybridization and Polymerase Chain Reaction Amplification. Endocrinology. vol.134, No. 3: 1305-1309.
Ayala M., Balint R.F., Fernandez-de-Cossio M.E., Canaan-Haden L., Larrick J.W., Gavilondo J.V. (1995) New Primer Strategy Improves Precision of Differential Display. Biotechniques. vol. 18, No. 5: 842-849.

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