Method of identifying non-host plant disease resistance genes

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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Details

C435S006120, C435S410000, C536S024100

Reexamination Certificate

active

07138273

ABSTRACT:
The invention describes a new method to isolate disease resistance genes in plants. The method teaches to transiently express in susceptible plants large numbers of R-gene homologs or non-host inducible genes isolated from non-host resistant plants. These plants can be screened for either disease resistance or ability to respond with a hypersensitive response to pathogen-elicitor subjection. The invention also reports several R-genes and non-host inducible genes that have been successfully isolated using the described method. These R-genes trigger a hypersensitive response in tobacco that is dependent on the presence of the ubiquitousP. infestanselicitor INF1. The presented R-genes are predicted to be both the first R-genes isolated that confer resistance againstP. infestansand the first R-genes involved in non-host resistance.

REFERENCES:
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patent: WO 98/02545 (1998-01-01), None
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Gowda et al., NRSA-1: a resistance gene homolog expressed in roots of non-host plants following parasitism byStriga asiatica, The Plant Journal 20(2):217-230 (1999).
Kamoun et al., A Gene Encoding a Protein Elicitor ofPhytophthora infestansIs Down-Regulated During Infection of Potato,MPMI 10:13-20 (1997).
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