Method, reagent and kit for genotyping of human papillomavirus

Details Method, reagent and kit for genotyping of human papillomavirus Method, reagent and kit for genotyping of human papillomavirus

1998-05-30

2000-04-04

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 71, 435 21, 435 28, 536 231, 536 243, 536 2431, 536 2432, 536 2433, C12Q 170, C12Q 168, C07H 2102, C07H 2104

Patent

active

06045993&

ABSTRACT:
The sequence of human papillomavirus present in a sample is determined by amplifying a portion of the L1 open reading frame of human papillomavirus genome to form L1 amplicons containing plus and minus amplified strands using MY09 and MY11 amplification primers, and then determining the positions of at least the A bases in the antisense amplicon using a consensus sequencing primer which is shifted six bases with respect to the MY09 primer (MY09-6). This primer has the sequence ARRGGAWACT GATCWARDTC (Seq. ID No. 3)

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Husman, et al. The use of General Primers GP5 and GP6 elongated at their 3'J ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR. Gen Virology 1995. vol. 76, pp. 1057-1062 .

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