IN vitro sorting method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007100, C435S008000

Reexamination Certificate

active

07138233

ABSTRACT:
The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising of the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having a desired activity.

REFERENCES:
patent: 4801529 (1989-01-01), Perlman et al.
patent: 5503851 (1996-04-01), Hank et al.
patent: 5989892 (1999-11-01), Nishimaki et al.
patent: 6023540 (2000-02-01), Walt et al.
patent: 6184012 (2001-02-01), Neri et al.
patent: 6258858 (2001-07-01), Nakajima et al.
patent: 6266459 (2001-07-01), Walt et al.
patent: 6310354 (2001-10-01), Hanninen et al.
patent: 6489103 (2002-12-01), Griffiths et al.
patent: 6808882 (2004-10-01), Griffiths et al.
patent: 2001/0020011 (2001-09-01), Mathlowitz et al.
patent: 2002/0022038 (2002-02-01), Griffiths et al.
patent: 2002/0119459 (2002-08-01), Griffiths
patent: 2002/0155080 (2002-10-01), Glenn et al.
patent: 2003/0124586 (2003-07-01), Griffiths et al.
patent: 2004/0005594 (2004-01-01), Holliger et al.
patent: 2004/0253731 (2004-12-01), Holliger et al.
patent: 2005/0037392 (2005-02-01), Griffiths et al.
patent: 2005/0064460 (2005-03-01), Holliger et al.
patent: 2005/0079510 (2005-04-01), Berka et al.
patent: 2005/0164239 (2005-07-01), Griffiths et al.
patent: 19646372 (1997-06-01), None
patent: 0 579 347 (1994-01-01), None
patent: 1 482 036 (2004-12-01), None
patent: WO91/05058 (1991-04-01), None
patent: WO 93/03151 (1993-02-01), None
patent: WO93/08278 (1993-04-01), None
patent: WO94/16332 (1994-07-01), None
patent: WO 94/23738 (1994-10-01), None
patent: WO94/24314 (1994-10-01), None
patent: WO 94/26766 (1994-11-01), None
patent: WO95/11922 (1995-05-01), None
patent: WO 95/24929 (1995-09-01), None
patent: WO 96/34112 (1996-10-01), None
patent: WO96/40723 (1996-12-01), None
patent: WO 97/40141 (1997-10-01), None
patent: WO 97/47763 (1997-12-01), None
patent: WO 98/13502 (1998-04-01), None
patent: WO 98/23733 (1998-06-01), None
patent: WO 98/31700 (1998-07-01), None
patent: WO 98/34120 (1998-08-01), None
patent: WO98/37186 (1998-08-01), None
patent: WO 98/41869 (1998-09-01), None
patent: WO 00/04139 (2000-01-01), None
patent: WO 00/40712 (2000-07-01), None
patent: WO 01/18244 (2001-03-01), None
patent: WO 02/22869 (2002-03-01), None
patent: WO 02/103363 (2002-12-01), None
patent: WO 03/044187 (2003-05-01), None
patent: WO 04/069849 (2004-08-01), None
patent: WO 04/083443 (2004-09-01), None
Ibrahim SF, van den Engh G. 2003. High-speed cell sorting: fundamentals and recent advances.Curr Opin Biotechnol. Feb.;14(1):5-12.
Mattheakis, L.C. et al., (1994), “Anin vitropolysome display system for identifying ligands from very large peptide libraries”,Proc. Natl. Acad. Sci. USA,91:9022-9026.
Cull, M.G. et al., (1992), “Screening for receptor ligands using large libraries of peptides linked to the C terminus of thelacrepressor”,Proc. Natl. Acad. Sci. USA,89:1865-1869.
Clackson, T. et al., (1994), “In vitroselection from protein and peptide libraries”,Trends in Biotechnology,12:173-184.
Bass, S. et al., (1990), “Hormone Phage: An Enrichment Method for Variant Proteins With Altered Binding Properties”,PROTEINS: Structure, Function, and Genetics,8:309-314.
Joyce, G.F., (1994), “In vitroevolution of nucleic acids”,Structural Biology, 4:331-336.
Chapman, K.B. et al., (1994), “In vitroselection of catalytic RNAs”,Structural Biology,4:618-622.
Wick, R. et al., (1996), “Enzyme-containing liposomes can endogenously produce membrane-constituting lipids”,Chemistry&Biology,3:277-285.
Oberholzer, T. et al., (1995), “Polymerase chain reaction in liposomes”,Chemistry&Biology,2:677-682.
Chakrabarti, A.C. et al., (1994), “Production of RNA by a Polymerase Protein Encapsulated Within Phospholipid Vesicles”,J. Mol. Evol,.39:555-559.
Widersten, M. et al., (1995), “Glutathione Transferases with Novel Active Sites Isolated by Phage Display from a Library of Random Mutants”,J. Mol. Biol.,250:115-122.
Soumillion, P., (1994), “Selection of β-Lactamase on Filamentous Bacteriophage by Catalytic Activity”,J. Mol. Biol.,237:415-422.
Oberholzer, T. et al., (1995), “Enzymatic RNA replication in self-reproducing vesicles: an approach to a minimal cell”,Biochemical and Biophysical Research Communications,207(1):250-257.
Keij, J.F. et al., (1994), “High-Speed Photodamage Cell Sorting: An Evaluation of the ZAPPER Prototype”,Methods in Cell Biology,42:371-386.
Gold, L. et al., (1995), “Diversity of Oligonucleotide Functions”,Annu. Rev. Biochem.,64:763-797.
Warburton, B., (1993), “Microcapsules from Multiple Emulsions”,Royal Society of Chemistry,138:35-51.
Tuerk, C. et al., (1990), “Systematic Evoluation of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase”,Science,249:505-510.
Smith, G.P., (1985), “Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion Surface”,Science,228:1315-1317.
Green, R. et al., (1992), “Selection of a Ribozyme That Functions as a Superior Template in a Self-Copying Reaction”,Science,258:1910-1915.
Ellington, A.D. et al., (1990), “In Vitroselection of RNA molecules that bind specific ligands”,Nature,346:818-822.
McCafferty, J. et al., (1990), “Phage antibodies: filamentous phage displaying antibody variable domains”,Nature,348:552-554.
Moore, M.J., (1995), “Exploration by lamp light”,Nature,374:766-767.
Walde, P. et al., (1994), “Oparin's Reactions Revisited: Enzymatic Synthesis of Poly(adenylic acid) in Micelles and Self-Reproducing Vesicles”,J. Am. Chem. Soc.,116:7541-7547.
The International Search Report (PCT/GB98/01889).
Tawfik & Griffiths, (1998), “Man-Made Cell-Like Compartments for Molecular Evolution”,Nature Biotechnology,16: 652-656.
D.S. Tawfik, et al, (1997), “Efficient and selective p-nitrophenyl-ester-hydrolyzing antibodies elicited by a p-nitrobenzyl phosphonate hapten”,European Journal of Biochemistry,224: 619-626.
J. Hanes and A. Pluckthun, (1997), “In vitro selection and evolution of functional proteins by using ribosome display”,National Academy Science,94: 4937-4942.
Elgen at al. (1980).J. Theor. Biol.85: 407-411.
Elgen et al. (1991).Biochemistry30: 11005-11018.
The European Search Report for EP 04 07 7211.
Anarbaev, et al. (1998). Klenow Fragment and DNA Polymerase o-Primase Fromserva Calf Thymus in Water-in-Oil Microemulsions, Biochimica et Biophysica Acta, 1384:315-324.
Atwell et al. (1999). Selection for Improved Subtilgases by Phage Display, Proc. Natl. Acad. Sci. USA, 96:9497-9502.
Bauer, Johann (1999), Advances in Cell Separation: Recent Developments in Counterflow Centrifugal Elutriation and Continuous Flow Cell Separation, J. Chromotography 722:55-69.
Brody & Quake (1999). A Self-Assembled Microlensing Rotational Probe. Applied Physics Letters 74:144-146.
Demartis et al. (1999). A Strategy for the Isolation of Catalytic Activities from Repertoires of Enzymes Displayed on Phage, Academic Press Limited, Article No. jmbi. 1998.2476, pp. 617-633.
Dressman et al. (2003). Transforming Single DNA Molecules into Fluorescent Magnetic Particles for Detection and Enumeration of Genetic Variations, PNAS 100:8817-8822.
Gasperlin et al. (2000). Viscosity Prediction of Lipophillic Semisolid Emulsion Systems by Neural Network Modelling, Int'l J. Pharm., 196:37-50.
Gasperlin et al. (1994). The Structure Elucidation of Semisolid w/o Emulsion Systems Containing Silicone Surfactant, Int'l J. Pharm., 107:51-56.
Ghadessy et al. (2001). Directed Evolution of Polymerase Function by Compartmentalized Self-Replication, PNAS, 98:4552-57.
Griffiths & Tawfik (2003). Directed Evolution of an Extremely Fast Pho

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