Purification and activity assurance of precipitated heterologous

Details Purification and activity assurance of precipitated heterologous Purification and activity assurance of precipitated heterologous

1984-06-01

1985-04-23

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

424 85, 435 68, 435 70, 4351723, 435240, 435241, 435948, 436548, C07G 700

Patent

active

045129223

ABSTRACT:
A process for maintaining refractile proteins in solubilized form by replacing the strongly denaturing solution with a weakly denaturing solution.

REFERENCES:
patent: 4414150 (1983-11-01), Goeddel
patent: 4438032 (1984-03-01), Golde et al.
patent: 4462940 (1984-07-01), Hanisch et al.
Goeddel et al., Nature, vol. 281, pp. 544 to 548 (Oct. 18, 1979).
Kleid et al., Science, vol. 214, pp. 1125 to 1129 (Dec. 4, 1981).
Williams et al., Science 215, 687-688 (1982).
Goeddel et al., Proc. Natl. Acad. Sci. USA 76, 106-110 (1979).
Wetzel et al., Biochemistry 19, 6096-6104 (1980).
Wetzel et al., Gene 16, 63-71 (1981).
Ullmann et al., Biochem. Biophys. Res. Commun. 35, 35-42 (1969).

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