Methods and compositions for evolving hydrogenase genes

Details Methods and compositions for evolving hydrogenase genes Methods and compositions for evolving hydrogenase genes

2006-11-14

2006-11-14

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S189000

Reexamination Certificate

active

07135290

ABSTRACT:
The invention provides methods and compositions for engineering microbes to generate Hydrogen. Some methods of the invention involve recoding of hydrogenase genes followed by subjecting the recoded genes to annealing-based recombination methods. The invention further provides methods of mating organisms that are transformed with recoded and recombined hydrogenase genes with other organisms containing different genome sequences.

REFERENCES:
patent: 4476105 (1984-10-01), Greenbaum
patent: 5871952 (1999-02-01), Ghirardi et al.
patent: 6180406 (2001-01-01), Stemmer
Kosourov, Sustained hydrogen photoproduction by Chlamydomonas reinhardtii: Effects of culture parameters, Biotechnology and Bioengineering, Jun. 30, 2002, 731-40, 78(7).
Happe, Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii, Eur. J. Biochem. Feb. 2002, 1022-32, 269(3).
Zhang, Biochemical and morphological characterization of sulfur-deprived and H2-producing Chlamydomonas reinhardtii (green alga), Planta, Feb. 2002, 552-61, 214(4).
Ghirardi, Microalgae: a green source of renewable H2, Trends Biotechnol. Dec. 1, 2000, 506-11, 18(12).
Ahmann, Combinatorial Mutagenesis of a Bidirectional Hydrogenase in Chlamydomonas reinhardtii, Feb. 21, 2002, National Science Foundation Grant No. AWSFL008-DS3.

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