In Vitro biosynthesis of proteins in native conformation

Details In Vitro biosynthesis of proteins in native conformation In Vitro biosynthesis of proteins in native conformation

1996-10-30

2000-03-07

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435183, 4352891, C12P 2106, C12N 900, C12M 136

Patent

active

060338687

ABSTRACT:
A method for the in vitro biosynthesis of proteins in native conformation which improves the technique which is well known in the art by immobilizing on the surface of an affinity matrix at discrete locations a ligand for which the protein being synthesized has an affinity and adding to the reaction mixture said affinity matrix having said immobilized ligand so that each molecule that is in the process of folding into a functional protein molecule may bind an immobilized ligand and be kept separated from other protein molecules as the folding proceeds. The technique works with either a batch method or a continuous method.

REFERENCES:
patent: 4362669 (1982-12-01), Verlander et al.
patent: 4748124 (1988-05-01), Vogler
Scouten, W.H. et al. "Immobilized cell free protein synthesis system" Journal of Molecular Recognition (Oct. 1993), vol. 6, No. 1, p. 4.
Komar, A.A. et al. "Cotranslational heme binding to nascent globin chains" FEBS Letters (Jul. 1993), vol. 326, No. 1,2,3, pp. 261-263.
Denisov, I.G. "Thermal stability of proteins in intermolecular complexes" Biophysical Chemistry (Aug. 1992), vol. 44, pp. 71-75.
Kigawa, T. et al. "A continuous cell-free protein synthesis system for coupled transcription-translation" Journal of Biochemistry (1991), vol. 110, pp. 166-168
Spirin, A.S. et al. "A continuous cell-free translation system capable of producing polypeptides in high yield" Science (Nov. 1988), vol. 242, pp. 1162-1164.

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