Methods and compositions for the expression of biologically acti

Details Methods and compositions for the expression of biologically acti Methods and compositions for the expression of biologically acti

1992-07-02

1995-05-30

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 697, 4352523, 43525233, 4353201, 536 234, 536 232, 935 10, 935 14, 935 27, 935 38, 935 44, 935 47, C12N 902, C12N 1553, C12N 1562, C12N 1563

Patent

active

054200270

ABSTRACT:
Disclosed are methods and compositions for effecting bacterial expression of eukaryotic cytochrome P450 enzymes and fusion proteins comprising a eukaryotic P450 domain fused to a reductase enzyme domain in a biologically active form. Certain embodiments involve the expression of eukaryotic cytochrome P450.sub.17.alpha. -hydroxylase which is expressed in large amounts in an E. coli host in a biologically active form without the need for coexpression or admixture of a cytochrome P450 reductase. Methods and compositions are also disclosed for the construction of cytochrome P450 enzyme hybrids or fusion proteins, incorporating the N-terminal 9 amino acids from bovine 17.alpha.-hydroxylase, which will enable many eukaryotic cytochrome P450 enzymes or fusion proteins to be expressed in bacteria.

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