Process for the preparation of .alpha.-acetolactic acid

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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4353201, 4352523, 43525232, 4352529, 4352534, 435885, C12P 1900, C12N 1500, C12N 100

Patent

active

054200210

ABSTRACT:
The invention relates to a process for the production of .alpha.-acetolactate and/or diacetyl, wherein a recombinant micro-organism containing an .alpha.-acetolactate synthase-encoding sequence is incubated in a medium containing an .alpha.-acetolactate precursor. The invention also provides a recombinant vector comprising a nucleotide sequence coding for an enzyme, which vector upon transfer into a host micro-organism enables expression of the nucleotide sequence in the host micro-organism, wherein the enzyme has .alpha.-acetolactate synthase activity.

REFERENCES:
patent: 4828994 (1989-05-01), Fahnestock et al.
Goelling, et al., "Cloning and Expression of an A-Acetolactate Decarboxylas Gene from Streptococcus lactis subsp. diacetylactis in Escherichia coli", Appl. Env. Microb., vol. 54, No. 7, Jul. 1988, pp. 1889-1891.
Falco et al Nucleic Acids Res. vol. 13 (11) pp. 4011-4027 issued 1985.
Glover Gene Cloning Chapman and Hall Ltd., New York, N.Y. 1984 Chapter 7 pp. 158-163.

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