Polynucleotides encoding Δ-endotoxins toxic to...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S279000, C800S260000, C536S023710, C424S093200, C435S071100, C435S418000, C435S252310, C435S320100, C435S252300

Reexamination Certificate

active

07022897

ABSTRACT:
Disclosed are novel polynucleotides encoding Lepidopteran- and Coleopteran-active δ-endotoxin polypeptides and compositions comprising the polypeptides. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise the novel nucleic acid segments encoding the δ-endotoxin polypeptides. Also disclosed are methods of identifying related polynucleotides encoding δ-endotoxin polypeptides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species.

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Donovan, et al., “Characterization of Two Genes EncodingBacillus thuringiensisInsecticidal Crystal Proteins Toxic to Coleoptera Species,” Appl. Environ. Microbiol., 58(12):3921-3927, 1992.
Donovan, et al., “Isolation and Characterization of EG2158, a New Strain ofBacillus thuringiensisToxic to Colepteran Larvae, and Nucleotide Sequence of the Toxin Gene,” Mol. Gen. Genet., 214(3):365-372, 1988.
English and Slatin, “Mode of Action of Delta-Endotoxins fromBacillus thuringiensis:A Comparison with other Bacterial Toxins,” Insect Biochem. Mol. Biol., 22:1-7, 1992.
Gallo, et al., Insecticidal Effectiveness ofMammea americana(Guttiferae) Extracts on Larvae of DiabroticaVirgifera virgifera(Coleoptera:Chrysomelidae) andTrichoplusia ni(Lepidoptera:Noctuidae)—Economic Botany 50(2) pp. 236-242 (1996).

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