Mass production and long-term preservation of fungivorous nemato

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing

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424 937, A01N 6300

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active

060336587

ABSTRACT:
A host fungus of a fungivorous nematode is inoculated on a solid medium or an artificial liquid medium containing an industrial vegetable waste or by-product, and then the nematode whose whole body have been sterilized is inoculated and mass-cultivated. Fungivorous ability of the nematode can be kept by subculturing using different host fungus on every culturing stage. The nematodes, when maintained about 10 days in an aerobic condition at 20-25.degree. C. with a relative humidity gradually inclined from high to low and dried to anhydrobiotic conditions, can be preserved for a long time. The nematode can be used for biological control of soil pathogens and soil insect pests.

REFERENCES:
patent: 5554533 (1996-09-01), Bedding et al.
Caubel et al., "Control of Rhizoctonia solani Kuhn, parasitic oncotton, with the nematode Aphelenchus avenae Bastian", Revue Nematol., 1981, 4(1), pp. 93-98.
Mankau et al., "Multiplication of Aphelenchus avenae on phytopathogenic soil fingi", Phytopatology, 1962, vol. 52, p. 741.
Townshend, Fungus Hosts of Aphelenchus Avenae Bastian, and Bursaphelechus Fungivorus Franklun & Hooper, 1962 and their attractiveness to these nematode species, Canadian Journal of Microbiology, Mar. 24, 1964, pp. 727-737.
Choi et al. "Propagation of Five Aphelechus avenae Isolates on Six Species of Fungi and Five Substrates" Japan Journal of Nematology, vol. 19, Dec. 1989, pp. 13-17.
Ishibashi et al. "Biological Control of Soil Pests by Mixed Application of Entomophathogenic and Fungivorous Nematodes" Journal of Nematology 23 (2) 1991 pp. 175-181.
Ishibashi "Integrated Control of Insect Pests by Steinernema carpocapsae" ed. Bedding et al., CSIRO, East Melbourne, Australia, pp. 105-113, 1994.
Ishibashi "Detection of Beneficial Nematodes in Japan and Systematized Nematode Application" Research Project on Biological Control Supported by Grant-in-Aid for Developmental Scientific Research, Ministry of Education, Culture and Science, Japan, Mar. 1993.

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