Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Reexamination Certificate
2006-05-30
2006-05-30
Olsen, Kaj K. (Department: 1753)
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
C204S454000
Reexamination Certificate
active
07052589
ABSTRACT:
Method and materials to carry out preparative-scale electrophoretic separations based on the principle of dynamically created non-co-directional effective electrophoretic mobilities are disclosed. The primary application areas of the method are in the separation, purification, enrichment, concentration or conditioning of both small and large molecular weight, weak and strong electrolyte compounds, such as pharmaceuticals, oligo- and polypeptides, proteins, oligonucleotides, etc. These objectives can be achieved based on the use of a secondary chemical equilibrium, alone or in combination with multiple protic and other secondary chemical equilibria. Though such electrophoretic operations could be achieved by other means, such as by conventional zone electrophoresis or isotachophoresis, the method disclosed here can provide greater separation power, simplicity and higher production rates.
REFERENCES:
Wan et al, J. Chromatog. A, 704, pp. 179-193 (1995).
Bauer et al. “Sodium Chloride in Preparative Free-Flow Cell Electrophoresis: Recent Developments” Electrophoresis, vol. 17, pp. 526-528, 1996.
Bauer et al. “Interval Carrier Free Electrophoresis For High Resolution Protein Purification” J Dispersion Science and Technology, vol. 19, pp. 937-950, 1998.
Bier et al. “A New Buffering System and Its Use in Electrophoresis and Isoelectric Focusing” Electrophoresis, vol. 14, pp. 1011-1018, 1993.
Bondy et al. “Sodium Chloride in Separation Medium Enhances Cell Compatibility of Free Flow Electrophoresis” Electrophoresis, vol. 16, pp. 92-97, 1995.
Burggraf et al. “Free Flow-Isoelectric Focusing of Human Cellular Lysates as Sample Preparation for Protein Analysis” Electrophoresis, vol. 16, pp. 1010-1015, 1995.
Cai et al. “A Family of Single-Isomer Chiral Resolving Agents for Capillary Electrophoresis. 3. Heptakis(2,3-dimethyl-6-sulfato)-β-cyclodextrin” Analytical Chemistry, vol. 70, pp. 580-589, 1998.
Cai et al. “Capillary Electrophoretic Separation of Weak Base Enantiomers Using the Single-Isomer Heptakis-(2,3 Dimethyl-6-sulfacto)-β-Cyclodextrin as Resolving Agent and Methanol as Background Electrolyte Solvent” J. Pharm. Biomded. Anal., vol. 18, pp. 615-621, 1998.
Canut et al. “Separation of Plant Membranes by Electromigration Techniques” J. Chromatography B, vol. 722, pp. 121-139, 1999.
Chankvetadze, B. “Capillary Electrophoresis in Chiral Analysis” John Wiley & Sons, New York, Table of Contents Only, 1997.
Glukhovskiy et al., “Analytical- and Preparative-Scale Isoelectric Focusing Separation of Enantiomers” Analytical Chemistry vol. 71, pp. 3814-3820, 1999.
Glukhovskiy, et al. “Preparative-Scale Isoelectric Focusing Separation of Enantiomers Using a Multicompartment Electrolyzer with Isoelectric Membranes” Electrophoresis, vol. 21, pp. 762-766, 2000.
Guiochon, G, et al. “Fundamentals of Preparative and Nonlinear Chromatography” Academic Press, New York, Table of Contents Only, 1994.
Hoffmann et al. “Separation and Purification of Methadone Enantiomers by Continuous- and Interval-Flow Electrophoresis” Analytical Chemistry, vol. 71, pp. 1840-1850, 1999.
Juza et al. “Simulated Moving-Bed Chromatography and Its Application to Chirotechnology” Trends in Biotech vol. 18, pp. 108-118, 2000.
Krivankova et al. “Continuous Free-Flow Electrophoresis” Electrophoresis, vol. 19, pp. 1064-1074, 1998.
Lanz, M. et al. “Enantiomeric Separation of methadone by Cyclodextrin-Based Capillary and Recycling Isotachophoresis” Electrophoresis, vol. 19, pp. 1081-1090, 1998.
Nath et al. “Separation of Enzymes From Microorganism Crude Extracts by Free-Flow Zone Electrophoresis” Biotechnology and Bioengineering, vol. 51, pp. 15-22, 1996.
Rhodes et al. PElectrohydrodynamic Effects in Continuous Flow Electrophoresis Applied and Theoretical Electrophoresis vol. 2, pp. 87-98, 1991.
Stalcup, A. et al. “Application of Classical Gel Electrophoresis to the Chiral Separation of Milligram Quantities of Terbutaline” Analytical Chemistry, vol. 70, pp. 144-148, 1998.
Taker et al. “Nonaqueous Capillary Electrophoretic Separation of Basic Enantiomers Using Heptakis(2,3-dimethyl-6-sulfato)-β-cyclodextrin” Electrophoresis vol. 20, pp. 2794-2798, 1999.
Glukhovskiy et al. “Use of Single-Isomer, Multiply-Charged Chiral Resolving Agents for the Continuous, Preparative-Scale Electrophoretic Separation of Enantiomers Based on the Principle of Equal-But-Opposite 6Analyte Mobilities,” Electrophoresis vol. 21, pp. 2010-2015, 2000.
Vincent et al. “A Family of single-Isomer Chiral Resolving Agents for Capillary Electrophoresis. 1. Heptakis(2,3-diacetyl-6-sulfato)-β-cyclodextrin” Analytical Chemistry, vol. 69, pp. 4226-4233, 1997.
Vincent et al. “A Family of Single-Isomer Chiral Resolving Agents for Capillary Electrophoresis. 2. Hepta-6-sulfato-β-cyclodextrin” Analytical Chemistry vol. 69, pp. 4419-4428, 1997.
Vincent et al. “Nonaqueous Capillary electrophoretic Separation of Enantiomers Using the Single-Isomer Heptakis(2,3-diacetyl-6-sulfato)-β-cyclodextrin as Chiral Resolving Agent” J. Chromatography A, vol. 816, pp. 233-241, 1998.
Vincent et al. “Systematic Approach to Methods Development for the Capillary Electrophoretic Analysis of a Minor Enantiomer Using a Single-Isomer Sulfated Cyclodextrin. A Case Study of L-Carbidopa Analysis” J. Chromatography A, vol. 817, pp. 105-111, 1998.
Weber et al. “Optimized Continuous Flow Electrophoresis” Electrophoresis, vol. 17, pp. 1906-1910, 1996.
Weber et al. “Counterbalancing Hydrodynamic Sample Distortion Effects Increases Resolution of Free-Flow Zone Electrophoresis” Electrophoresis, vol. 19, pp. 1104-1109, 1998.
Weber et al. “Recent Developments in Preparative Free Flow Isoelectric Focusing” Electrophoresis, vol. 19, pp. 1649-1653, 1998.
Weber et al. “Interval Isotachophoresis for Purification and Isolation of Ionogenic Species” Electrophoresis, vol. 19, pp. 3090-3093, 1998.
Weber et al. “Stability of Continuous Flow Electrophoresis” Electrophoresis, vol. 19, pp. 3094-3095, 1998.
Weber et al. “Application of Binary Buffer Systems to Free Flow Cell Electrophoresis” Electrophoresis, vol. 21, pp. 325-328, 2000.
William et al., “The Use of Hydrodynamic Counterflow to Improve the Resolution and Detection of the Minor Component in the Capillary Electrophoretic Analysis of Enantiomers.” Enantiomer vol. 1, pp. 183-191, 1996.
Williams et al. “Dry Look at the CHARM (Charged Resolving Agent Migrationl) Model of Enantiomer Separations by Capillary Electrophoresis”, J. Chromatography A vol. 777, pp. 295-309, 1997.
Zhu et al. “A Family of Single-Isomer, Sulfated γ-Cyclodextrin Chiral Resolving Agents for Capillary Electrophoresis. 1. Octakis(2,3-diacetyl-6-sulfato)-γ-cyclodextrin” Analytical Chemistry, vol. 72, pp. 310-317, 2000.
Baker & Botts L.L.P.
Olsen Kaj K.
The Texas A&M University System
LandOfFree
Method for electrophoretic separations using dynamically... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for electrophoretic separations using dynamically..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for electrophoretic separations using dynamically... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3585260