Enkurin and uses thereof

Details Enkurin and uses thereof Enkurin and uses thereof

2006-01-31

2006-01-31

Le, Long V. (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007950, C435S176000, C436S514000, C436S536000, C436S015000

Reexamination Certificate

active

06991909

ABSTRACT:
A novel protein, enkurin, that is preferentially expressed in sperm has been discovered. Enkurin binds to TRPCs including TRPC2-S, a protein encoded by TRPC2 that is not predicted to be a calcium channel subunit. The invention includes methods of identifying compounds that affect enkurin expression or activity, and are useful, e.g., for contraception and treatment of infertility.

REFERENCES:
Brann et al., “Type-specific inositol 1,4,5-trisphosphate receptor localization in the vomeronasal organ and its interaction with a transient receptor potential channel, TRPC2,”J. Neurochem.83(6):1452-60 (2002).
Ives et al., “Characterization of chromosomal DNA amplifications with associated tetracycline resistance inBacillus subtilis,” J. Bacteriol.172(9):4936-44 (1990).
Yidirim et al., “The mouse C-Type transient receptor potential 2 (TRPC2) channel: alternative splicing and calmodulin binding to its N terminus,”Proc. Natl. Acad. Sci U S A.100(5):2220-2225 (2003).
Arnoult et al., “ZP3-dependent Activation of Sperm Cation Channels Regulates Acrosomal Secretion During Mammalian Fertilization,”J. Cell Biol.134:637-645 (1996).
Arnoult et al., “Activation of mouse sperm T-type Ca2+channels by adhesion to the egg zona pellucida,”Proc. Nat. Acad. Sci. USA93:13004-13009 (1996).
Arnoult et al., “Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation,”Proc. Natl. Acad. Sci. USA96:6757-6762 (1999).
Clapham et al., “The TRP Ion Channel Family,”Nature Rev. Neurosci.2:387-396 (2001).
Clark et al., “Caltrin, the Calcium Transport Regulatory Peptide of Spermatozoa, Modulates Acrosomal Exocytosis in Response to the Egg's Zona Pellucida,”J. Biol. Chem.268:5309-5316 (1993).
Florman et al., “An Adhesion-Associated Agonist from the Zona Pellucida Activites G Protein-Promoted Elevations of Internal Ca2+and Ph that Mediate Mammalian Sperm Acrosomal Exocytosis,”Dev. Biol.135:133-146 (1989).
Florman et al., “Activation of Voltage-Dependent Calcium Channels of Mammalian Sperm Is Required for Zona Pellucida-Induced Acrosomal Exocytosis,”Dev. Biol.152:304-314 (1992).
Florman et al., “Sequential Focal and Global Elevations of Sperm Intracellular Ca2+Are Initiated by the Zona Pellucida during Acrosomal Exocytosis,”Dev. Biol.165:152-164 (1994).
Gallouzi et al., “Delineation of mRNA Export Pathways by the Use of Cell-Permeable Peptides,”Science294:1895-1901 (2001).
Genebank Accession No. AF111107.
Genebank Accession No. AF111108.
Genebank Accession No. NM_145010.
Hofmann et al., “Cloning, expression and subcellular localization of two novel splice variant of mouse transient receptor potential channel 2,”Biochem. J.351:115-122 (2000).
Jungnickel et al., “Trp2 regulates entry of Ca2+into mouse sperm triggered by egg ZP3,”Nature Cell Biology3:449-502 (2001).
Kirkman-Brown et al., “Biphasic Elevation of [Ca2+]iin Individual Human Spermatozoa Exposed to Progesterone,”Dev. Biol.,222:326-335 (2000).
Liman et al., “TRP2: A candidate transduction channel for mammalian pheromone sensory signaling,”Poc. Natl. Acad Sci. USA96:5791-5796 (1999).
O'Toole et al., “Ca2+Entry through Store-operated Channels in Mouse Sperm is Initiated by Egg ZP3 and Drive the Acrosome Reaction,”Molecular Biol. Of Cell11:1571-1584 (2000).
Son et al., “Acrosome reaction of human spermatozoa is mainly mediated by α1H T-type calcium channels,”Mol. Human Reprod.6:893-897 (2000).
Vannier et al., “Mouse trp2, the homologue of the human trpc2 pseudogene, encodes mTrp2, a store depletion-activated capacitative Ca2+entry channel,”Proc. Natl. Acad. Sci. USA96:2060-2064 (1999).
Wes et al., “TRPCI, a human homolog of aDrosophilastore-operated channel,”Proc. Natl. Acad. Sci. USA92:9652-9656 (1995).
Wissenbach et al., “Structure and mRNA expression of a bovine trp homologue related to mammalian trp2 transcripts,”FEBS Lett.429:61-66 (1998).

Affiliated with

Also associated with

Rating

Enkurin and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enkurin and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enkurin and uses thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3579037