Composition for inoculating legumes and method therefor

Plant protecting and regulating compositions – Plant growth regulating compositions – Micro-organisms or from micro-organisms

Reexamination Certificate

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C435S252200

Reexamination Certificate

active

07022649

ABSTRACT:
A method for preparing a concentrated inoculating composition which contains a universal use culture medium which is sterilized and cooled, then added to Rhizobium Japonicum and then allowed to multiply for 46 hours. This is followed by the addition to the culture medium of powdered maltose, liquid maltose and lactose saccharides as well as the fungicide potassium sorbate. The general use culture medium can comprise previously treated peat as one of its components. The above results in an inoculating composition for use with leguminous plants with or without peat incorporated as part of the general use culture medium.

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Kuykendall et al., “Rhizobium japonicum derivatives differing in nitrogen-fixing efficiency and carbohydrate utilization,” Applied and Environmental Microbiology 32(4):511-519, 1976.
Bergey's Manual of Determinative Microbiology, 8th Ed., R.E. Buchanan & N.E. Gibbons, eds., pp. 262-264, The Williams & Wilkins Co., Baltimore, 1974.
ATCC Bacteria and Bacteriophages, 19th Ed., P. Pienta et al, eds., pp. 72 and 301, American Type Culture Collection, Rockville, MD, 1996.

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