Oligonucleotides for detecting tubercle bacillus and method...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C536S023100, C536S023700

Reexamination Certificate

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07105319

ABSTRACT:
An oligonucleotide, capable of specific cleavage and amplification of a pab gene which codes for pab, an antigenic protein ofMycobacterium tuberculosis, or an RNA derived therefrom, as well as being useful in high sensitive detection and identification thereof, is provided. Further, a combination of oligonucleotides, useful in specific amplification, high sensitive detection and identification of an RNA derived fromMycobacterium tuberculosispab gene as well as a process for detectingMycobacterium tuberculosisby using said combination, are provided.

REFERENCES:
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patent: EP 1 002 878 (2000-05-01), None
patent: WO 00/75371 (2000-12-01), None
patent: WO 03/010309 (2003-02-01), None
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CD-ROM containing an unbridged electronic version of the article on the “Preparation and Utilization of Isolated and Purified Oligonucleotides” by Dr. Andrew Chin.
Francesca Mariani, et al., “Mycobacterium tuberculosisH37Rv comparative gene-expression analysis in synthetic medium and human macrophage”, Gene, Elsevier Biomedical Press., vol. 253, No. 2, XP-004215732, Aug. 8, 2000, pp. 281-291.
G. Zambardi, et al., “Comparison of three primer sets for the detection ofMycobacterium tuberculosisin clinical samples by polymerase chain reaction”, Annales de Biologie Clinique, vol. 51, No. 10-11, XP-009050704, 1993, pp. 893-897.
E.A. Sweet-Cordero, et al., “Burden ofMycobacterium tuberculosisin sputum samples can be reliably determined using a quantitative, non-radioactive polymerase chain reaction assay”, Tubercle and Lung Disease, Churchill Livingstone Medical Journals, vol. 77, No. 6, XP-004540712, Dec. 1996, pp. 496-501.

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