Method for identifying phosphoprotein

Details Method for identifying phosphoprotein Method for identifying phosphoprotein

2006-07-11

2006-07-11

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S068100, C435S195000, C356S344000, C204S450000

Reexamination Certificate

active

07074583

ABSTRACT:
A method for identifying phosphoprotein, which comprises: subjecting a sample protein to two-dimensional electrophoresis; dephosphorylating the sample protein with phosphatase; once again performing the two-dimensional electrophoresis under the same conditions; and detecting the spot that migrates to the alkaline side of the isoelectric focusing as the phosphoprotein is provided as a convenient method for identifying phosphoprotein with high accuracy.

REFERENCES:
Robert W. Leggett et al., The Journal of Biological Chemistry, Oct. 1995, vol. 270, No. 43, p. 25882.
M. Butler et al., Journal of Neurochemistry, 1986, vol. 47, No. 5, pp. 1520, 1521.

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