Method and arrangement for scanning microscopic specimens...

Radiant energy – Photocells; circuits and apparatus – Photocell controls its own optical systems

Reexamination Certificate

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C250S234000

Reexamination Certificate

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06852964

ABSTRACT:
The present invention discloses a method and an arrangement for scanning microscopic specimens (15) with a scanning device. The microscopic specimen (15) is displaceable on a specimen stage (35) in at least two spatial directions. A light beam (3) scans the specimen (15) within a defined scan field (52) by way of a scanning module (7), and the light (17) proceeding from the specimen is detected. A PC (34) is also provided for analysis and calculation. The scan field (52) is defined in such a way that it incompletely encompasses a specimen region that is to be examined. Means (23, 31) are provided which displace the specimen stage (35) in such a way that the entire specimen region of interest can be covered by the plurality of resulting scan fields (521, 522, . . . 52n). The data of the individual scan fields (521, 522, . . . 52n) detected from the specimen region being examined are assembled in the PC (34) into an overall image.

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patent: WO 96 377 97 (1996-11-01), None
Werner Zuschratter et al. “Acquisition of multiple image stacks with a confocal laser scaning” in Proceedings of SPIE vol. 3261, pp. 177-188.
Georg Erich Steiner, et al., Automated data acquisition by confocal laser scanning microscopy and image analysis of triple stained immunofluorescent leukocytes in tissue, Journal of Immunological Methods 237 (2000) 39-50 Apr. 1999.

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