Method and apparatus for rapid change of fluorescence bands...

Details Method and apparatus for rapid change of fluorescence bands... Method and apparatus for rapid change of fluorescence bands...

2005-02-22

2005-02-22

Gagliardi, Albert (Department: 2878)

Radiant energy

Luminophor irradiation

Methods

C250S339010, C250S339020, C356S317000, C356S319000, C356S326000

Reexamination Certificate

active

06858852

ABSTRACT:
A method is disclosed for operation of an image-generating optical system for detection of characteristic quantities of the wavelength-dependent behavior of an illuminated specimen, such as the emission behavior and/or absorption behavior and, in particular, the fluorescence and/or luminescence and/or phosphorescence and/or enzyme-active light emission and/or enzyme-active fluorescence, for the operation of a laser scanning microscope, comprising the steps of splitting the image point information of the specimen into spectral components in a spatially resolved manner on the detection side in dependence on wavelength and carrying out at least one summing for different spectral components.

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Article: “Resolution of multiple green fluorescent protein color variants and dyes using two-photon microscopy and imaging spectroscopy” Journal of Biomedical Optics Jul. 2001 vol. 6. No. 3 pp. 311-318.
*English Abstract of DE 198 01 139.
*English Abstract of DE 198 29 944.

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