Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Reexamination Certificate
2005-06-28
2005-06-28
Kruse, David H (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
C435S468000
Reexamination Certificate
active
06911575
ABSTRACT:
Methods and compositions for altering a nucleotide sequence of interest in a plant is provided. The method involves introducing a chimeric oligonucleotide into a plant cell, wherein the oligonucleotide comprises at least two intervening blocks of RNA residues with intervening DNA residues. The RNA blocks are homologous to a plant nucleotide sequence. The chimeric oligonucleotide is capable of folding to form a duplex oligonucleotide. The chimeric oligonucleotide is maintained within the nucleus of the plant whereby the alteration is introduced into the target sequences of the plant genome.
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Baszczynski Christopher L.
Bowen Benjamin A.
Duesing John H.
Peterson David J.
Tagliani Laura A.
Alston & Bird LLP
Kruse David H
Pioneer Hi-Bred International , Inc.
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