Isolation of membrane bound ligand-specific complexes

Details Isolation of membrane bound ligand-specific complexes Isolation of membrane bound ligand-specific complexes

2005-11-01

2005-11-01

Saunders, David (Department: 1644)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007230, C435S007240, C435S007800, C435S379000, C435S820000, C436S501000, C436S503000, C436S063000, C436S086000, C436S087000, C436S161000, C436S173000, C436S177000

Reexamination Certificate

active

06960447

ABSTRACT:
A method to recover receptors without removing microenvironmental components associated with them is described. The membranes containing the receptors are first associated with ligand-coupled solid supports to achieve association of the membranes through a receptor/ligand complex which includes the microenvironment to the solid support and then removing the membrane from the receptor and its microenvironment by shear forces. The thus isolated receptors and their microenvironments can then be analyzed for their role in cellular differentiation, apoptosis, transformation and the like.

REFERENCES:
patent: 3843324 (1974-10-01), Edelman et al.
patent: 5721120 (1998-02-01), Seifert et al.
patent: 8403151 (1984-08-01), None
patent: WO 94/25487 (1994-11-01), None
Edelman et al, Methods in Enzymology, 34, 195-225, 1974.
Jakobovits et al, Biochem. Biophys Research Communications, 100, 1484-1490, 1981.
Kupchik ,In John (Ed.), Monoclonal Hybridoma Antibodies, Techniques and Applications, CRC Press, Inc., 1982, pp. 81-89.
Chevallet et al., Electrophoresis (1998) 19:1901-1909.
Gallagher et al., “The Red Blood Cell Membrane” in Beutler et al., Eds., Williams Hematology, pp. 333-343 (6th ed. 2001).
Guyton & Hall, Eds., Textbook of Medical Physiology, pp. 382-391 (10th ed. 2000).
Link et al., Nature Biotechnol. (1999) 17:676-682.
Molloy et al., Electrophoresis (1998) 19:837.
Neubauer et al., Proc. Natl. Acad. Sci. USA (1997) 94:385-390.
Rudert et al., Biotechnol. Ann. Rev. (2000) 5:45-86.
Rutishauser et al., Proc. Natl. Acad. Sci. (1973) 70(12)3894-3898.
Parker et al., Journal of Biological Chemistry (1984) 259(15):9906-9912.
Supplementary European Search Report for EP 02756793.2 mailed on Aug. 3, 2004, 2 pages.

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