Methods and vectors for site-specific recombination in plant...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

Reexamination Certificate

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C800S298000, C800S300000, C800S317300, C435S320100, C435S419000, C536S023100

Reexamination Certificate

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06849778

ABSTRACT:
Novel compositions and methods useful for genetic engineering of plant cells are provided. In particular, plastid constructs comprising recombining sites are provided. Such constructs find use in methods for site specific recombination in plant cell plastids. Plant cells and plants comprising the constructs described herein, as well as plant cells and plants produced by the methods of the present invention are of interest.

REFERENCES:
patent: WO 9741228 (1997-11-01), None
patent: WO 0121768 (2001-03-01), None
Birch, R.G. Plant Transformation: Problems and Strategies for Practical Application; Annu Rev. Plant Physiol. Plant Mol. Biol. 1997, 48:297-326.*
Odell et al; Use of site-specific recombination systems in plants; 1994. In J Paszkowski, Ed, Homologous Recombination in Plants. Kluewer Academic Publishers, Dordrecht, The Netherlands, pps 219-270.*
Kilby, et al, 1993, Trends in Genetics 9: 413-421.*
Svab, et al, (Proc. Natl. Acad. Sci. USA, vol. 90, pp. 913-917, 1993).*
Dale, et al, (Proc. Natl. Acad. Sci. USA, vol. 88, pp. 10558-10562, 1991.*
Dale E.C. et al, “Gene transfer with subsequent removal of the selection gene from the host genome,” PNAS USA, National Academy of Science (Washington), p. 10558-62, (Dec. 1991).
Dale E.C. et al, “Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase,” Gene, Elsevier (Amsterdam), 9: p. 79-85, (1990).

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