Methods for designing inhibitors of serine/threonine kinases...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C530S300000, C435S069100, C435S071100

Reexamination Certificate

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06849716

ABSTRACT:
The invention relates to methods for designing inhibitors of serine/threonine kinases and tyrosine kinases, particularly MAP kinases, through the use of ATP-binding site mutants of those kinases. The methods of this invention take advantage of the fact that the mutant kinases are capable of binding inhibitory compounds of other kinases with greater affinity than the corresponding wild-type kinase. The invention further relates to the mutant kinases themselves and crystallizable co-complexes of the mutant kinase and the inhibitory compound.

REFERENCES:
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patent: WO 9302209 (1993-02-01), None
patent: WO 9835048 (1998-08-01), None
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Wilson et al., “The Structural Basis for the Specificity of Pyridinylimidazole Inhibitors of p38 MAP Kinase,”Chem.&Biol.4, 423-431 (1997).
Gum et al., “Acquisition of Sensitivity of Stress-Activated Protein Kinases to the p38 Inhibitor, SB 203580, by Alteration of One or More Amino acids within the ATP Binding Pocket,”J. Biol. Chem.273, 15605-15610 (1998).
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