Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-05-03
2005-05-03
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C204S450000, C204S451000, C204S456000
Reexamination Certificate
active
06887668
ABSTRACT:
The invention provides novel electrophoretic methods for high-resolution separation and high-sensitivity detection of nucleic acids. The methods involve the use of a high-resolution buffer and a counter-migrating high-affinity intercalating dye in an electrophoresis system to achieve superior separation and detection of nucleic acids. A kit for separation and detection of nucleic acids in a sample by electrophoresis is also provided. The kit comprises a gel buffer containing a high-resolution intercalating dye with a resolution of at least 2.0 between the 271-bp and 282-bp φX 171 HAIII nucleic acid fragments nucleic acid fragments; and a high-affinity intercalating dye having a positive charge and DNA affinity constant of at least 106M−1.
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http://www.cstl.nist.gov/biotech/strbase/pub_pres/Butler2004a_pre.pdf.*
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Chen Fu-Tai-A
Liu Ming-Sun
Beckman Coulter Inc.
Fredman Jeffrey
Hogan & Hartson LLP
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