Method for enhancing cellobiase activity of termitomyces...

Details Method for enhancing cellobiase activity of termitomyces... Method for enhancing cellobiase activity of termitomyces...

2005-09-20

2005-09-20

Marx, Irene (Department: 1651)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S171000, C435S244000, C435S155000

Reexamination Certificate

active

06946277

ABSTRACT:
This invention provides a method for enhancing the cellobiase activity of the strainTermitimyces clypeatususing 2-deoxy-D-glucose as glycosylation inhibitor, said process comprises inoculating and growing mycelial culture of (the edible mushroom)Termitimyces clypeatus, in sterilized medium containing 0.05 to 5.0% of 2-deoxy-D-glucose in addition to 0.5 to 2.0% of cellobiose, succinate—0.5%, 2 to 3% of ammonium di hydrogen phosphate and conventional micro-nutrients at pH between 3 to 8 and incubating at temperatures between 20-35° C. under shaking in aerobic conditions, and separating the culture medium by known methods, and using the culture filtrate directly as the source of the enzyme cellobiase and also for endo-glucanase and cellobiohydrolase for use in cellulose hydrolysis.

REFERENCES:
Kubicek et al., Canadian Journal of Microbiology, vol. 33, pp. 698-703.
Bhattacharyya et al., “Purification and characterization of an extracellular beta-xylosidase fromTermitomyces clypeatus”, Biotechnology Progross 13 (6): 822-825 (1997).
Roy et al., “Development of high molar mass cellobiase comples by spontaeous protein-protein interaction in the culture filtrate ofTermitomyces clypeatus”, Folia Microbiologica 39 (6): 463-70 (1994).
Sengupta et al., “Purification and Characterization of a beta glucosidase cellobiase fromTermitomyces clypeatus”, Biochim. Biophys. Acta 1076 (2): 215-20 (1991).

Affiliated with

Also associated with

Rating

Method for enhancing cellobiase activity of termitomyces... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for enhancing cellobiase activity of termitomyces..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for enhancing cellobiase activity of termitomyces... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3449964