Cell cultures

Details Cell cultures Cell cultures

1975-08-20

1977-05-03

Rosen, Sam

Textiles: fiber preparation

C12K 900

Patent

active

040213021

ABSTRACT:
Cell culture comprising a novel cell strain in suitable nutrient medium, wherein the novel cell strain is produced by disaggregated feline embryo tissue cell and serially passaging the same from about the 20th to about the 48th passage level, so as to be cultured and subcultured with the resultant cell strains remaining substantially diploid, typically fibroblastic and substantially free from morphological transformations. Subculturing is facilitated by treatment of a confluent sheet of the disaggregated cells chelating agent before each transfer into a new batch of nutrient medium.

REFERENCES:
patent: 3255080 (1966-06-01), Emery
patent: 3264187 (1966-08-01), Slater
patent: 3293130 (1966-12-01), Slater et al.

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