Antimicrobial protein from Lyophyllum shimeji

Details Antimicrobial protein from Lyophyllum shimeji Antimicrobial protein from Lyophyllum shimeji

2005-04-19

2005-04-19

Carlson, Karen Cochrane (Department: 1653)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

Reexamination Certificate

active

06881720

ABSTRACT:
The object of the present invention is to screen and identify a novel antimicrobial protein which can inhibit the growth of plant pathogenic microorganisms at a relatively low concentration such asPyricularia oryzaeandRhizoctonia solanicausative of two major diseases causing damage to rice crops and, further, to clone the gene of this protein. According to the present invention, an antimicrobial protein which can be obtained from a fraction of an aqueous extract ofLyophyllum shimejiprecipitated by the ammonium sulfate precipitation method, has an antimicrobial activity at least againstRhizoctonia solaniorPyricularia oryzae, and shows the presence of components of about 70 kDa and/or about 65 kDa in molecular weight in the SDS-PAGE method. A gene encoding this protein and a method of using the same are provided.

REFERENCES:
patent: 5712139 (1998-01-01), Nishimura et al.
patent: 6291648 (2001-09-01), Kawamura et al.
patent: 6559294 (2003-05-01), Griffais et al.
patent: WO9411511 (1994-05-01), None
patent: WO9504754 (1995-02-01), None
Lazar E, Watanabe S, Dalton S, Sporn MB. Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities. Mol Cell Biol. Mar. 1988;8(3):1247-52.*
Bowie Ju, Reidhaar-Olson JF, Lim Wa, Sauer Rt. Deciphering the message in protein sequences: tolerance to amino acid substitutions. Science. Mar. 16, 1990;247(4948):1306-10.*
Qun Zhu et al., Bio/Technology, vol. 12 (1994) pp. 807-812.
Willie Lin et al., Bio/Technology, vol. 13 (1995) pp. 686-691.
Franky R.G. Terras et al., The Plant Cell, vol. 7 (1995) pp. 573-588.
Shigeru Oita et al., Biosci. Biotech. Biochem, vol. 60, No. 3 (1996) pp. 481-483.
Franky R.G. Terras et al., J. of Bio. Chem., vol. 267, No. 22 (1992) pp. 15301-15309.
Angela Schlumbaum et al., Nature, vol. 324 (1986) pp. 365-367.
Karen Broglie et al., Science, vol. 254 (1991) pp. 1194-1197.
Felix Mauch et al., Plant Physiol., vol. 88 (1988) pp. 936-942.
Marianne B. Sela-Buurlage et al., Plant Physiol., vol. 101 (1993) pp. 857-863.

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