Endonuclease of immune cell, process for producing the same...

Details Endonuclease of immune cell, process for producing the same... Endonuclease of immune cell, process for producing the same...

2005-04-19

2005-04-19

Navarro, Mark (Department: 1645)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

C435S195000, C435S199000, C530S350000

Reexamination Certificate

active

06881561

ABSTRACT:
The present invention relates to a novel endonuclease enzyme which is secreted from immune cell and recognizes bacterial DNA as foreign agent and processes it to produce about 10 bp single-stranded oligonucleotide including CpG motif which is involved in immune response. Also, the present invention relates to a process for producing the endonuclease which comprises culturing human B-lymphoblastic IM9 cell line or TPA-treated myelogenous U937 cell line on an appropriate medium to produce the said endonuclease and isolating the said endonuclease from the cell lysate or the culture medium. In addition, the present invention relates to an immune adjuvant comprising about 10 bp single-stranded oligonucleotide having CpG motif produced by treatment of bacterial DNA by endonuclease.

REFERENCES:
patent: WO9602555 (1996-02-01), None
Kwon et al (International Journal of Biochem & Cell Biology (vol. 30) pp 217-223, Feb. 1998.*
NATURE, vol. 374, No. 6522, Apr. 6, 1995, A.M. Krieg et al: “CpG motifs in bacterial DNA trigger direct β-cell activation”, pp. 546-549.
SCIENCE, vol. 273, Jul. 19, 1996, Y. Sato et al: “Immunostimulatory DNA Sequences Necessary for Effective Intradermal Gene Immunization”, pp. 352-354.

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