Method for the detection of dyes in fluorescence microscopy

Optics: measuring and testing – By dispersed light spectroscopy – Utilizing a spectrometer

Reexamination Certificate

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C359S368000, C356S317000, C250S459100, C250S339010, C250S339020

Reexamination Certificate

active

06891613

ABSTRACT:
A method for optical detection of characteristic quantities of the wavelength-dependent behavior of an illuminated specimen, such as the emission behavior and/or absorption behavior, preferably the fluorescence and/or enzyme fluorescence comprises determining at least one spectral centroid and/or a maximum of the emission radiation and/or of the absorbed radiation. An arrangement for performing the method is disclosed.

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Article: “Resolution of multiple green fluorescent protein color variants and dyes using two-photon microscopy and imaging spectroscopy” Journal of Biomedical Optics Jul. 2001 vol. 6. No. 3 pp. 311-318.

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