Antimicrobial theta defensins and methods of using same

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C424S185100, C514S009100

Reexamination Certificate

active

06890537

ABSTRACT:
The present invention relates to an isolated cyclic peptide, theta defensin, having antimicrobial activity, and to theta defensin analogs. A theta defensin can have the amino acid sequence Xaa1-Xaa2-Xaa3-Xaa4-Xaa5-Xaa1-Xaa6-Xaa4-Xaa4-Xaa1-Xaa1-Xaa6-Xaa4-Xaa5-Xaa1-Xaa3-Xaa7-Xaa5, wherein Xaa1 to Xaa8 are defined; wherein Xaa1 can be linked through a peptide bond to Xaa8; and wherein crosslinks can be formed between Xaa3 and Xaa3, between Xaa5 and Xaa5, and between Xaa7 and Xaa7. For example, the invention provides a theta defensin having the amino acid sequence Gly-Phe-Cys-Arg-Cys-Leu-Cys-Arg-Arg-Gly-Val-Cys-Arg-Cys-Ile-Cys-Thr-Arg (SEQ ID NO:1), wherein the Gly at position 1 (Gly-1) is linked through a peptide bond to Arg-18, and wherein disulfide bonds are present between Cys-3 and Cys-16, between Cys-5 and Cys-14, and between Cys-7 and Cys-12. The invention also relates to antibodies that specifically bind a theta defensin and to isolated nucleic acid molecules encoding a theta defensin. In addition, the invention relates to methods of using theta defensin or a theta defensin analog to reduce or inhibit microbial growth or survival in an environment capable of sustaining microbial growth or survival by contacting the environment with the theta defensin.

REFERENCES:
patent: 4543252 (1985-09-01), Lehrer et al.
patent: 4659692 (1987-04-01), Lehrer et al.
patent: 4705777 (1987-11-01), Lehrer et al.
patent: 5242902 (1993-09-01), Murphy et al.
patent: 5324716 (1994-06-01), Selsted et al.
patent: 5422424 (1995-06-01), Selsted et al.
patent: 5459235 (1995-10-01), Selsted et al.
patent: 5464823 (1995-11-01), Lehrer et al.
patent: 5547939 (1996-08-01), Selsted
patent: 5731149 (1998-03-01), Selsted et al.
patent: 5804558 (1998-09-01), Lehrer et al.
patent: 5821224 (1998-10-01), Selsted et al.
patent: 5840498 (1998-11-01), Selsted et al.
patent: 5844072 (1998-12-01), Selsted et al.
patent: 5916872 (1999-06-01), Chang et al.
patent: WO 9616075 (1996-05-01), None
patent: WO 9708199 (1997-03-01), None
patent: WO 9911663 (1999-03-01), None
patent: WO 9913080 (1999-03-01), None
Ahmad et al., “Liposomal entrapment of the neutrophil-derived peptide indolicidin endows it with in vivo antifungal activity,”Biochem. Biophys. Acta., 1237:109-114 (1995).
Bals et al., “Mouse β-Defensin 1 Is a Salt-Sensitive Antimicrobial Peptide Present in Epithelia of the Lung and Urogenital Tract,”Infect. Immun., 66:1225-1232 (1998).
Blond et al., “The cyclic structure of microcin J25, a 21-residue peptide antibiotic form ofEscherichia coli,”Eur. J. Biochem., 259:747-755 (1999).
Derua et al., “Analysis of the Disulfide Linkage Pattern in Circulin A and B, HIV-Inhibitory Macrocyclic Peptides,”Biochem. Biophys. Res. Comun., 228:632-638 (1996).
Galvez et al., “Purification and Amino Acid Composition of Peptide Antibiotic AS-48 Produced byStreptococcus(Enterococcus)faecalissubsp.liquefaciensS-48,”Antimicrob. Agents Chemother., 33:437-441 (1989).
Goldman et al., “Human β-Defensin-1 Is a Salt-Sensitive Antibiotic in Lung That Is Inactivated in Cyctic Fibrosis,”Cell, 88:553-560 (1997).
Gustafson et al., “Circulins A and B: Novel HIV-Inhibitory Macrocyclic Peptides from the Tropical TreeChassalia parvifolia,”J. Amer. Chem. Soc., 116:9337-9338 (1994).
Lehrer and Ganz, “Antimicrobial peptides in mammalian and insect host defence,”Current Opinion Immunol. 11:23-27 (1999).
Lehrer et al., “Defensins: Endogenous Antibiotic Peptides of Animal Cells,”Cell, 64:229-230 (1991).
Smith et al., “Cystic Fibrosis Airway Epithelia Fail to Kill Bacteria Because of Abnormal Airway Surface Fluid,”Cell, 85:229-236 (1996).
Tang and Selsted, “Characterization of the Disulfide Motif in BNBD-12, and Antimicrobial β-Defensin Peptide from Bovine Neutrophils,”J. Biol. Chem., 268:6649-6653 (1993).
Valore et al., “Human β-Defensin-1: An Antimicrobial Peptide of Urogenital Tissues,”J. Clin. Invest., 101:1633-1642 (1998).
Wade et al., “All-D amino acid-containing channel-forming antibiotic peptides,”Proc. Natl. Acad. Sci. USA, 87:4761-4765 (1990).
Wu et al., “Proteintrans-splicing by a split intein encoded in a split DnaE gene ofSynechocystissp. PCC6803,”Proc. Natl. Acad. Sci. USA, 95:9226-9231 (1998).
Zanetti et al., “Cathelicidins: a novel protein family with a common proregion and a variable C-terminal antimicrobial domain,”FEBS Lett., 347:1-5 (1995).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Antimicrobial theta defensins and methods of using same does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Antimicrobial theta defensins and methods of using same, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antimicrobial theta defensins and methods of using same will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3412342

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.