DNA encoding Bacillus licheniformis PWD-1 keratinase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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43525233, 4352525, 4353201, 536 232, C12N 956, C12N 120, C12N 1500, C07H 2104

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active

057121478

ABSTRACT:
An isolated DNA encoding a keratinase is disclosed. The isolated DNA may be any of (a) isolated DNA which encodes the Bacillus licheniformis PWD-1 keratinase enzyme of FIG. 1, (b) isolated DNA which hybridizes to an oligonucleotide probe, which hybridizes to the DNA of (a) above, and does not hybridize to DNA encoding the Bacillus licheniformis NCIB 6816 subtilisin Carlsberg serine protease, and (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in nucleotide sequence due to the degeneracy of the genetic code, and which encodes a keratinase enzyme.

REFERENCES:
patent: 4908220 (1990-03-01), Shih et al.
patent: 4959311 (1990-09-01), Shih et al.
patent: 5063161 (1991-11-01), Shih et al.
patent: 5171682 (1992-12-01), Shih et al.
patent: 5186961 (1993-02-01), Shih et al.
Jacobs et al. (1985) Nucleic Acids Research 13(24); 8913-8926.

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