Method to screen phage display libraries with different ligands

Details Method to screen phage display libraries with different ligands Method to screen phage display libraries with different ligands

2005-01-25

2005-01-25

Ponnaluri, Padmashri (Department: 1639)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S005000, C435S006120, C435S320100, C435S091500, C435S091500, C435S091500, C435S091500, C530S388100, C536S025300

Reexamination Certificate

active

06846634

ABSTRACT:
The invention provides a method for selecting, from a repertoire of polypeptides, a population of functional polypeptides which bind a target ligand in a first binding site and a generic ligand in a second binding site, which generic ligand is capable of binding functional members of the repertoire regardless of target ligand specificity, comprising the steps of: a) contacting the repertoire with the generic ligand and selecting functional polypeptides bound thereto; and b) contacting the selected functional polypeptides with the target ligand and selecting a population of polypeptides which bind to the target ligand. The invention accordingly provides a method by which a polypeptide repertoire is preselected, according to functionality as determined by the ability to bind the generic ligand, and the subset of polypeptides obtained as a result of such preselection is then employed for further selection according to the ability to bind the target ligand.

REFERENCES:
patent: 6057098 (2000-05-01), Buechler et al.
Cook and Tomlinson (1995)Immunol. Today, 16: 237.
Corbett et al. (1997)J. Mol. Biol., 268: 69.
Ravetch et al. (1981)Cell, 27: 583.
Schäble and Zachau (1993)Biol. Chem. Hoppe-Seyler, 374: 1001.
Williams et al. (1996)J. Mol. Biol., 264: 220.
Kawasaki et al. (1997)Genome Res., 7: 250.
Hieter et al. (1982)J. Biol. Chem., 257: 1516.
Vasicek and Leder (1990)J. Exp. Med., 172: 609.
Chothia and Lesk (1987)J. Med. Biol., 196; 901.
Chothia et al. (1989)Nature, 342: 877.
Chothia et al. (1989)Nature, 227: 799.
Tomlinson et al. (1995)EMBO J., 14: 4628.
Williams et al. (1996)J. Mol. Biol., 264: 220.
Martin et al. (1996)J. Mol. Biol., 263: 800.
Shirai et al. (1996)FEBS Letters, 399: 1.
Tomlinson et al. (1996)J. Mol. Biol., 256: 813.
Ignatovich et al. (1997)J. Mol. Biol.,268: 69.
Smith (185)Science, 228: 1315.
Scott and Smith (1990)Science, 249: 386.
McCafferty et al. (1990)Nature, 348: 552.
Marks et al (1991)J. Mol. Biol., 222: 581.
Hoogenboom & Winter (1992)J. Mol. Biol, 227:381.
Nissim et al. (1994)EMBO J., 13: 692.
Griffiths et al. (1994)EMBO J.., 13: 3245.
DeKruif et al. (1995)J. Mol. Biol., 248: 97.
Barbas et al. (1992)Proc. Natl. Acad. Sci. USA, 89: 4457.

Affiliated with

Also associated with

Rating

Method to screen phage display libraries with different ligands does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method to screen phage display libraries with different ligands, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method to screen phage display libraries with different ligands will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3410293