Process for the preparation of D-pantothenic acid and/or...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S041000, C435S183000, C435S193000, C435S252300, C435S252320, C435S320100, C530S350000, C536S023200

Reexamination Certificate

active

06913912

ABSTRACT:
A process for the preparation of D-pantothenic acid and/or salts thereof or feedstuffs additives comprising these by fermentation of microorganisms of the Enterobacteriaceae family, in particular those which already produce D-pantothenic acid, in which the nucleotide sequence(s) in the microorganisms which code(s) for the adk gene is/are enhanced, in particular over-expressed.

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M. Brune, et al., Nucleic Acids Research, vol. 13, No. 19, pp. 7139-7151, XP-001033922, “Cloning and Sequencing of the Adenylate Kinase Gene (ADK) ofEscherichia coli”, Oct. 11, 1995.
Blattner, et al., “The Complete Genome Sequence ofEscherichia coliK-12”, Science, vol. 277, Sep. 5, 1997, pp. 1453-1462.
Holmes, et al., “Purification and Characterization of Adenylate Kinase as an Apparent Adenosine Triphosphate-dependent Inhibitor of Ribonuclease II inEscherichia coli,” The Journal of Biological Chemistry, vol. 248, No. 6, Mar. 25, 1973, pp. 2014-2021.

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