Human lysophospholipase

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C536S022100, C536S023100

Reexamination Certificate

active

06838245

ABSTRACT:
The present invention provides a human lysophospholipase (NHLP) and polynucleotides which identify and encode NHLP. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. In addition, the invention provides methods for producing NHLP and for treating or preventing disorders associated with expression of NHLP.

REFERENCES:
patent: 5773276 (1998-06-01), Portilla et al.
Biolabs Catalog p. 61, 1986.*
Sugimoto, H. et al., “Purification, cDNA Cloning, and Regulation of Lysophospholipase from Rat Liver.”J. Biol. Chem.(1996) 271(13):7705-7711.
Selle, H. et al., “Glycerophosphocholine release in human erythrocytes. 1H spin-echo and 31P-NMR evidence for lysophospholipase.”Eur. J. Biochem.(1993) 212(2):411-416.
Endresen, M.J. et al., “Increased lipolytic activity of sera from pre-eclamptic women due to the presence of a lysophospholipase.”Scand.J.Clin.Lab Invest.(1993) 53(7):733-739.
Anderson, R. et al., “Alpha-tocopherol prevents cyclosporin A-mediated activation of phospholipase A2 and inhibition of Na+, K(+)-adenosine triphosphatase activity in cultured hamster renal tubular cells.”Toxicol.Appl.Pharmacol.(1994) 125(2):176-183.
Sugimoto, H. et al. (GI 1552244), GenBank Sequence Database (Accession D63885), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894.
Sugimoto, H. et al. (GI 1552243), GenBank Sequence Database (Accession D63885), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894.
Sugimoto, H. et al. “Purification, characterization, and inhibition by phosphatidic acid of lysophospholipase transacylase from rat liver.”J.Biol.Chem.(1994) 269(8):6252-6258.
GenBank Accession R12332 “yf55e02.r1Homo sapienscDNA clone 25773 (5′)”, Submitted by R.K. Wilson (1995).
DbEST record ID “yf55e02.r1Homo sapienscDNA clone 25773 (5′)” Record corresponds to Genbank Accession R12332 submitted by R.K. Wilson (1996).
Genbank Accession R59445 “yh17f05.r1Homo sapienscDNA clone 37830 (5′)” Submitted by R.K. Wilson (1995).
DbEST record ID “yh17f05.r1Homo sapienscDNA clone 37830 (5′)” Record corresponds to Genbank Accession R12332 submitted by R.K. Wilson (1996).
Boguski, M.S. et al., “Gene Discovery in dbESt”,Science, 265: 1993-1995 (1994).
Yan, Y.P. et al., “Molecular cloning and functional expression of human deoxyhypusine synthase cDNA based on expressed sequence tag information”,Biochem. J., 315: 429-434 (1996).
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Garsetti, D. et al., “Butyric acid-induced differentiation of HL-60 cells increases the expression of a single lysophospholipase”,Biochem J., 288: 831-837 (1992).
Weltzien, H.U., “Cytolytic and Membrane-Perturbing Properties of Lysophosphatidylcholine”,Biochimica et Biophysica Acta, 559: 259-287 (1979).
Wang, A. et al., “Regiospecificity and Catalytic Triad of Lysophospholipase I”,J. Biol. Chem., 272: 22030-22036 (1997).
Wang, A. et al., “Cloning, Expression, and Catalytic Mechanism of Murine Lysophospholipase I”,J. Biol. Chem., 272: 12723-12729 (1997).
She, H.S., et al., “The substrate specificities of four different lysophospholipase as determined by a novel flourescence assay”,Biochm J., 298: 23-29 (1994).
Taylor, J.K. et al., “Comparison of Intestinal Phospholipase A/Lysophospholipase and Sucrase-Isomaltase Genes Suggests a Common Structure for Enterocyte-Specific Promoters”,DNA and Cell Biology, 16: 1419-1428 (1997).
DATABASE EMBL, Accession No. U89352, Mar. 7, 1997, Wang, A. et al.: “Mus Musculus lysophospholipase I mRNA, complete cds,”.
DATABASE EMBL, Accession No. AA62396, Mar. 19, 1997, National Cancer Institute, Cancer Genome Anatomy Project (CGAP): “zs16b08.r1 NCI—CGAPHomo sapienscDNA clone IMAGE: 685335 5′ similar to WP: K04G2.5 CE06099 ESTERASE”.

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