DNA polymerases having amino acid substitutions and homologs...

Details DNA polymerases having amino acid substitutions and homologs... DNA polymerases having amino acid substitutions and homologs...

2005-04-05

2005-04-05

Fredman, Jeffrey (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300, C536S024330, C530S350000

Reexamination Certificate

active

06875573

ABSTRACT:
Thermostable DNA polymerases both in native form and having single amino acid substitutions and optionally N-terminal deletions are disclosed. These polymerases exhibit a substantial improvement of DNA sequencing performance compared to Taq DNA polymerase. The instant DNA polymerases also possess improved salt tolerance.

REFERENCES:
patent: 5210036 (1993-05-01), Comb et al.
patent: 5837450 (1998-11-01), Dahlberg et al.
patent: 5948614 (1999-09-01), Chatterjee
patent: 0 902 035 (1999-03-01), None
patent: WO9429482 (1994-12-01), None
patent: WO9840496 (1998-09-01), None
patent: WO9947539 (1999-09-01), None
patent: WO9965938 (1999-12-01), None
patent: WO0114568 (2001-03-01), None
Foldes-Papp, Z., et al. “Fluorescent high-density labeling of DNA: error-free substitution for a normal nucleotide” Journal of Biotechnology, Elsevier Science Publishers, Amsterdam, NL vol. 86, No. 3, Apr. 13, 2001 pp. 237-253.
Reeve, M. A., et al. “A novel thermostable polymerase for DNA Sequencing” Nature, MacMillian Journals Ltd., London, GB vol. 376, Aug. 31, 1995 pp. 796-797.
Voss, H., et al. “Automated Cycle Sequencing with Taquenase: Protocols for Internal Labeling, Dye Primer and “Doublex” Simultaneous Sequencing” Biotechniques, vol. 23, No. 2, 1997 pp. 312-318.
Bolchakova, E. V., et al. “Comparison of the Properties of Different Eubacterial DNA Polymerases and Their Use in Fluorescent DNA Sequencing” International Genome Sequencing and Analysis Conference, vol. 12, 2000, p. 50 12th International Genome Sequencing and Analysis Conference: Miami Beach, Florida, USA Sep. 12-15, 2000.

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