Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2005-03-15
2005-03-15
LeGuyader, John L. (Department: 1635)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S006120, C435S325000, C435S375000, C536S023100, C536S024500, C530S326000, C530S322000
Reexamination Certificate
active
06867043
ABSTRACT:
The present invention provides a peptide that can complex with an oligonucleotide, e.g. an antisense oligonucleotide, and deliver it into a cell. The present invention also provides compositions comprising a complex of such a peptide and an oligonucleotide and methods of delivering an oligonucleotide into a cell and of inhibiting protein expression using such compositions. The present invention also provides a method of making such a complex, a method of sensitizing cells to anti-cancer agents such as paclitaxel, and pharmaceutical compositions.
REFERENCES:
patent: 5959096 (1999-09-01), Bennett
Tamm, I. et al. The Lancet. 2001, Aug. 358: 489-497.*
Agrawal et al., TIBTECH 1996. 14:376-380□□.*
Gewirtz et al., 1996. Proc. Natl. Acad. Sci. v 93, pp. 3161-3163□□.*
Braasch, D. A. Biochemistry. Apr. 2002; 41(14): 4503-4510□□.*
Branch, A. D., (1998).Trends Biochem Sci. Feb.; 1998 23(2):45-50□□.*
Oehlke et al. Cellular Uptake of Antisense Oligonucleotides After Complexing or Conjugation with Cell-penetrating Model Peptides,Eur. J. of Mol. Biol.(2002) 269 (16) :4025-32; and.
Okuda et al. Antisense Delivery into Mitochondria Using Peptide,Proceedings—28thInternational Symposium on Controlled Release of Bioactive Materials and 4thConsumer and Diversified products Conference(2001) 2:1237-1238.
De La Torre, B., Albericio, F., Saison-Behmoaras, E., Bachi, A., and Eritja, R. (1999)Bioconjugate Chem.,10:1005-1-12 (Exhibit 3).
Eritja, R., Pons, A., Escarceller, M., Giralt, E., and Albericio, R. (1991)Tetrahedron47:4113-4120 (Exhibit 4).
Neves, C., Byk, G., Scherman, D., and Wils, P. (1999)FEBS Lett.453:41-45 (Exhibit 5).
Morris, M.C., Vidal, P. Chaloin, L., Heitz, F., and Divita, G. (1997)Nucl. Acids Res.25:2730-2736 (Exhibit 6) and.
Pichon C, Roufai MB, Monsigny M, Midoux P. (2000) Histidylated oligolysines increase the transmembrane passage and the biological activity of antisense oligonucleotides.Nucl. Acids Res.28: 504-512 (Exhibit 8).
Benimetskaya Luba
Guzzo-Pernell Nancy
Stein Cy A.
Cooper & Dunham LLP
LeGuyader John L.
Schultz J D
The Trustees of Columbia University in the City of New York
White John P.
LandOfFree
Peptides that deliver antisense oligonucleotides which... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Peptides that deliver antisense oligonucleotides which..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Peptides that deliver antisense oligonucleotides which... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3395307