Method for labeling individual cells

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving fixed or stabilized – nonliving microorganism,...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S459000, C427S002130

Reexamination Certificate

active

06869772

ABSTRACT:
A method is provided for the labeling of individual cells. Labeling is accomplished by coating a particle with at least one dye or nucleic acid sequence encoding a marker protein. The particle is then propelled toward the cell resulting in the particle contacting the cell for a time sufficient for the dye or nucleic acid to leave the particle and enter the cell. The present method allows for the differential labeling of individual cells within dense populations of cells.

REFERENCES:
patent: 4326524 (1982-04-01), Drake, Jr. et al.
patent: 4945050 (1990-07-01), Sanford et al.
patent: 5036006 (1991-07-01), Sanford et al.
patent: 5100792 (1992-03-01), Sanford et al.
patent: 5179022 (1993-01-01), Sanford et al.
patent: 5204253 (1993-04-01), Sanford et al.
patent: 5371015 (1994-12-01), Sanford et al.
patent: 5436134 (1995-07-01), Haugland et al.
patent: 5466587 (1995-11-01), Fitzpatrick-McElligott et al.
patent: 5478744 (1995-12-01), Sanford et al.
patent: 5573909 (1996-11-01), Singer et al.
patent: 5656449 (1997-08-01), Yue
patent: 5723218 (1998-03-01), Haugland et al.
patent: 5849526 (1998-12-01), Pichersky
patent: 5888829 (1999-03-01), Gee et al.
patent: 6013486 (2000-01-01), Dellaporta
patent: 6290991 (2001-09-01), Roser et al.
patent: WO 0169244 (2001-09-01), None
Wissing et al. “Illumination of the Malaria Parasite Plasmodium falciparum Alters Intracellular pH” (2002) J. Biol. Chem., 277 (40), 37747-37755.*
Davey et al., “Flow Cytometry of Microorganisms-Chapter 4” (1993) pp. 1-9 and pp. 1-18 of bibliography, accessed on the Internet at: pcfcij.dbs.ac.uk/thesis/tchap4.html on Feb. 27, 2003.*
“Sigma-Aldrich Catalog—Fluorescent Probes” pp. 1-4, accessed on the Internet at: www.sigmaaldrich.com/Brands/Fluka_Riedel_Home/Analytical/Fluorescent_Probes/Product_vs_Methodology/Miscellaneous.html on Feb. 27, 2003.*
Gan Wen-Biao et al., “Multicolor “Diolistic” labeling of the nervous system using lipophilic dye combinations” Neuron. Aug. 2000. pp. 219-225. vol. 27, No. 2. ISSN: 0896-6273.
Wong W T et al., “Multicolor diolistic labeling of the nervous system using lipophilic dye combinations.” Society for Neuroscience Abstracts. Pgs. Abstract No. 125.10. vol. 26, No. 1-2, 2000. 30th Annual Meeting of the Society of Neuroscience; New Orleans, LA, USA; Nov. 04-09, 2000: ISSN: 0190-5295.
Kettunen P et al., “Rapid loading of calcium indicators by particle mediated ballistic delivery.” Society for Neuroscience Abstracts. p. 1008. vol. 27, No. 1. 2001; 31st Annual Meeting of the Society for Neuroscience; San Diego, California, USA, Nov. 10-15, 2001. ISSN: 0190-5295.
Betz et al., “Activity-dependent Fluorescent Staining and Destaining of Living Vertebrate Motor Nerve Terminals”,J. Neurosci., 1992, 12:363-75.
Chalfie et al., “Green Fluorescent Protein as a Marker for Gene Expression”,Science, 1994, 263:802-805.
Craig et al., “The Distribution of Glutamate Receptors in Cultured Rat Hippocampal Neurons: Postsynaptic Clustering of AMPA-Selective Subunits”Neuron, 1993, 10:1055-1068.
Dunaevsky et al., “Developmental regulation of spine motility in the mammalian central nervous system”,Proc. natl. Acad. Sci. USA, 1999, 96:13438-13443.
Gan and Macagno, “Developing Neurons Use a Putative Pioneer's Peripheral Arbor to Establish Their Terminal Fields”,J. Neurosci., 1995, 5:3254-3262.
Gan and Lichtman, “Synaptic Segregation at the Developing Neuromuscular Junction”,Science, 1998, 282:1508-1511.
Gan et al., “Vital imaging and ultrastructural analysis of individual axon terminals labeled by iontophoretic application of lipophilic dye”,Neurosci. Methods, 1999, 93:13-20.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for labeling individual cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for labeling individual cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for labeling individual cells will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3383470

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.