Method of amplifying or detecting HIV-1 RNA

Details Method of amplifying or detecting HIV-1 RNA Method of amplifying or detecting HIV-1 RNA

2005-04-19

2005-04-19

Whisenant, Ethan (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S022100, C536S023740

Reexamination Certificate

active

06881544

ABSTRACT:
A simple, speedy and sensitive method of detecting HIV-RNA using an oligonucleotide which can bind to an intramolecularly free region of the genomic RNA of HIV-1 at relatively low and constant temperatures (at 35° C. to 50° C., preferably at 41° C.) as an oligonucleotide primer for use in amplification of a nucleic acid.

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T. Kievits, et al., Journal of Virological Methods, vol. 35, No. 3, XP-000576430, pp. 273-286, NASBA ™ Isothermal Enzymatic in Vitro Nucleic Acid Amplification Optimized for the Diagnosis of HIV-1 Infection.
S. Bruisten, et al., Aids Research and Human Retroviruses, vol. 9, No. 3, XP-002932546, pp. 259-266, “Detection of HIV-1 Distrubution in Different Blood Fractions by Two Nucleic Acid Amplification Assays”, 1993.
J. W. Romano, et al., Clinics in Laboratory Medicine, vol. 16, No. 1, XP-000600141, pp. 89-103, “NASBA a Novel Isothermal Detection Technology For Qualitative and Quantitative HIV-1 RNA Measurements”, Mar. 1, 1996.

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