Process for producing des(64,65)-proinsulin

Details Process for producing des(64,65)-proinsulin Process for producing des(64,65)-proinsulin

1989-12-07

1992-07-14

Elmore, Carolyn

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

530303, C12P 2106

Patent

active

051302365

ABSTRACT:
This application describes a process for producing des(64,65)-human proinsulin from human proinsulin by treating the latter with trypsin and carboxypeptidase B. The process is conducted by applying any or all of the following parameters: (1) use of an alkylated or acylated trypsin; (2) a temperature of from about 0.degree. C. to about 10.degree. C.; (3) a pH of from about 8 to about 10; and (4) use of a buffer selected form the group consisting of ammonium bicarbonate and glycine.

REFERENCES:
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patent: 4569792 (1984-06-01), Frank et al.
Chevallier, Sur. J. Biochem., 5 (1968) 480-486.
Given et al., J. Clin. Invest. 76, 1398-1405 (1985).
Peavy et al., J. Biol. Chem. 260, 13989-13994 (1985).

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