Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1985-02-07
1990-09-25
Teskin, Robin L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
530350, 530351, 530324, 530333, 4351723, 435320, 4352523, 43525233, 435 91, 536 27, 514 2, 514 8, 514 12, 424 852, 424 856, C12P 2100, C12P 1934, C12P 2122
Patent
active
049593145
ABSTRACT:
Muteins of biologically active proteins such as IFN-.beta. and IL-2 in which cysteine residues that are not essential to biological activity have been deleted or replaced with other amino acids to eliminate sites for intermolecular crosslinking or incorrect intramolecular disulfide bridge formation. These muteins are made via bacterial expression of mutant genes that encode the muteins that have been synthesized from the genes for the parent proteins by oligonucleotide-directed mutagenesis.
REFERENCES:
patent: 4518584 (1985-05-01), Mark et al.
patent: 4816566 (1989-03-01), DeChiara et al.
Itakura et al., Science, vol. 198, pp. 1056-1063, Dec. 9, 1977, Expression in Escherichia coli of a Chemically Synthesized Gene for the Lormone Somatostatr.
Zolter et al. Methods in Enzymology, vol. 100, pp. 468-500, 1983, "Oligonocteotide-Directed Mutagenesis of DNA Fragments Cloned Into M13 Vectors".
Lin Leo S.
Lu Shi-Da Yu
Mark David F.
Wang Alice M.
Cetus Corporation
Halluin Albert P.
McLaughlin Jane R.
Murashige Kate H.
Teskin Robin L.
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