Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of culturing cells in suspension
Patent
1994-12-22
1998-01-13
Beisner, William H.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of culturing cells in suspension
435394, 435403, 435410, 4354301, 435243, 4352891, 4352865, 4352991, C12M 136, C12N 502
Patent
active
057078688
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention relates to a device for culturing cellular biological material in the form of solid particles in the presence of a liquid culture medium. In this instance, "solid particles of cellular biological material" is understood to mean particles consisting, in all or in part, of cellular biological material, the other part, if appropriate, consisting of inert material such as a substrate or immobilization material. It can be, for example, cell clusters or parts of plant tissues such as pieces of roots or alternatively isolated cells coated onto inert substrates. As the said particles are generally fine, although not necessarily so, the device of the invention can, in fact, constitute a device for cell culturing in liquid medium, the said particles then comprising cells. This device contains an enclosure intended to be completely filled with the said liquid and to contain the said solid particles in contact with the liquid. It thus comprises means for supplying and discharging the liquid of the enclosure and means for retaining the said solid particles and for separating from the liquid in order to keep the solid particles within the enclosure in a specific region and optionally means for circulating and treating the liquid outside the enclosure. Another subject of the present invention is a process for carrying out the reaction of the particles with the liquid, especially a process for culturing cells in liquid medium using such a device.
BACKGROUND OF THE INVENTION
A reactor of this type has been described in International Patent Application WO 86 05202. However, the device described previously did not have a variable volume. Specifically, in certain cases, such as the culturing of plant somatic embryos, it appears that it can be advantageous, indeed necessary, to carry out culturing at a constant density by volume of cells in order to have correct development of the embryos or even to reduce this density by increasing the volume of the culturing enclosure, partly due to the release of certain compounds during maturation of the embryos, it being possible for these compounds to have a stimulating or inhibiting effect on the maturation.
Moreover, the reactor described in the abovementioned patent application contains, close to the culturing region, a helix (28) intended to create circulation of the liquid medium, such that it keeps the solid particles or cells in the condition of a fluidized bed. However, this arrangement includes the risk of injuring or detrimentally affecting the particles or cells, in particular if the latter escape from the fluidized bed, for example in the case of disturbance to or disfunctioning of the system.
SUMMARY OF THE INVENTION
An aim of the present invention is to provide a bioreactor which makes it possible to carry out culturing of cellular biological material at a constant cell density by volume during the development of the culture and, additionally, overcomes the disadvantages of the abovementioned reactor.
In order to do this, the present invention provides a device for culturing cellular biological material in the form of solid particles in which the said solid particles are brought into contact with a liquid culture medium, making it possible to keep the density by volume of the said cellular biological material substantially constant with respect to the volume of the said culture medium, containing: which at least one of the said walls (1) can be movable so that the enclosure has a variable volume, and from the enclosure, and liquid in order to keep the particles within the enclosure, characterized in that it contains means, consisting of at least one of the said movable walls (1) of the enclosure, for varying the volume of the culture medium in the culture chamber, especially in order to increase the volume of the culture medium during the development of the culture and the increase in the volume of the cellular biological material.
In an embodiment of the device according to the invention, the enclosure defines, at least i
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"Biogasanlagen: Nutzung des Mikrobiellen Lebensraums bie der Entsorgung" Stadlbauer et al, Chemiker-Zeitung, 108, Jan. 1984.
Boulay Michel
Deloire Alain
Mauro Marie-Claude
Meybeck Alain
Pierry Guy
Beisner William H.
I.V.M.H. Recherche
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