Ribozymes

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 9151, 4352523, 4353201, 435325, 435410, 536 245, C12P 1934, C07H 2102, C12N 1509, C12N 510

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057078351

ABSTRACT:
Compounds having highly specific endoribonuclease activity are described. The compounds of this invention, also known as ribozymes, comprise ribonucleotides having two hybridizing regions with predetermined sequences capable of hybridizing with a plant, animal or viral target RNA, a region of defined sequence and a base paired stem region.

REFERENCES:
patent: 4987071 (1991-01-01), Cech et al.
patent: 5254678 (1993-10-01), Hasseloff et al.
Christoffersen et al. "Ribozymes as human therapeutic agents." J. Med. chem 38:2023-2037, Jun. 1995.
Crystal. "Transfer of genes to humans: Early lessons and obstacles to success." Science 270: 404-410, Oct. 1995.
Stull et al. "Antigen, ribozyme and aptamer nucleic acid drugs: Progress and prospects." Pharm. Res. 12: 465-483, Apr. 1995.
"Report and recomendations of the panel to assess the NIH investment in research on gene therapy" Orkin and Motulsky, co-chairs. National Institutes of Health, Dec. 1995.
Dreyfus, D.H., (1988). Restriction Ribozymes? The Einstein Quart. Journal of Biology and Medicine, 6: 92-93.
Jeffries, A., (1986) Thesis. Self-Cleavage of ASBV: Deletion Analyses and Cleavage In Trans. Department of Biochemistry, The University of Adelaide, Adelaide, South Austrialia 5001.
Koizumi, M. et al., (1988). Cleavage of specific RNA by designed ribozymes. Febs Letters, 239: 285-288.
Maddox, J., (1989). The Great Gene Shears Story. Nature, 342:609-613.
Perriman, R. et al., (1992). Extended taget-site specificity for a hammerhead ribozyme. Gene, 113: 157-163.
Sullivan et al., (1987). Meeting in RNA processing. Abstract of Cold Spring Harbor, 163: back cover.
Takayama, K. and Inouye, M., (1988). Antisense RNA as a Tool For Regulation of Gene Expression. 8th Int. Biothechnology Symp., (Pt. 1): 97-112.
Zoumadakis, M. et al., (1994). The influence of imperfectly paired helices I and III on the catalytic activity of hammerhead ribozymes. Nucleic Acids Research, 22: 5271-5278.
Been, M.D. et al., (1986) "One Binding Site Determines Sequence Specificity of Tetrahymena Pre-rRNA Self-Splicing, and RNA Enzyme Activity," Cell 47: 207-216.
Boehm, S., (1987), "Similarities between a predicted secondary structure for the M.sub.1 RNA ribozyme and the tRNA binding center of 16S rRNA from E. coli"FEBS Letters, 220: 283-287.
Breuning, G. et al., (1987) "Non-Enzymatic Cleavage and Ligation of a Plant Satellite RNA," Plant Molecular Biology 140: 495-502.
Breuning, G. et al., (1988A) "Replication of Small Satellite RNAs and Viroids: Possible Participation of Nonenzymic Reactions," RNA Gentics II: 127-145.
Breuning, G. et al., (1988B) "Small RNA Autolytic Cleavage and Ligation," Structure and Expression 1: 239-248.
Breuning, G. (1989) "Compilation of Self-Cleaving Sequences from Plant Virus Satellite RNAs and Other Sources", Methods in Enzymology 180: 546-558.
Buzayan, J. et al., (1986) "Non-Enzymatic Cleavage and Ligation of RNAs Complementary to a Plant Virus Satellite RNA," Nature 323: 349-353.
Buzayan, J. et al., (1988A) "RNA Mediated Formation of A Phosphorothioate Diester Bond," Biochemical and Biophysical Research Communication 156: 340-347.
Buzayan, J. et al., (1988B) "Autolytic Processsing of a Phosphorothioate Diester Bond," Nucleic Acids Research 16(9): 4009-4023.
Cech, T.R., (1987) "The Chemistry of Self-Splicing RNA Enzymes", Science 236; 1532-1539.
Chuat, J. et al., (1989) "Can Ribozymes Be Used To Regulate Procaryote Gene Expression?", Biochemical and Biophysical Research Communications 162: 1025-1029.
Cotten, M. et al., (1989) "Ribozyme Mediated Destruction of RNA in vivo" The EMBO Journal 8: 3861-3866.
Eckner, R. et al., (1991) "Mature mRNA 3' End Formation Stimulates RNA Export from the Nucleus" The EMBO Journal 10: 3513-3522.
Forster, A.C. et al., (1988) "Self-Cleaving Viroid and Newt RNAs May Only Be As Dimers" Nature 334: 265-267.
Forster, A.C. and Symons R.H., (1987A) "Self-Cleavage of Plus and Minus RNAs of a Virusoid and a Structural Model for Active Sites" Cell 49: 211-220.
Forster, A.C. et al., (1987B) "Self-Cleavage of Virusoid Is Performed by the Proposed 55-Nucleotide Active Site" Cell 50: 9-16.
Gerlach, W. et al., (1986) "Satellite Tabacco Ringspot Virus RNA: Biological Acitivity of DNA Clones and Their in Vitro Transcripts," Virology 151: 172-185.
Guerrier-Takada, C. et al., (1983) "The RNA Moiety of Ribonuclease P Is the Catalytic Subunit of the Enzyme," Cell 35: 849-857.
Hampel, A. et al., (1988) "A Model for the RNA Catalyzed Cleavage of a Plant Satellite RNA," Journal of Cellular Biochemistry 12D: 31.
Haseloff, J. and Gerlach, W.L., (1989) "Simple RNA enzyme with new and highly specific endoribonuclease activities" Nature 334: 585-591.
Huillier, A. et al., Ribozyme mediated suppression of .alpha.lactalbumin Expressed in C1271 Cells by T7/Vaccinia Virus (Abstract from conference proceedings).
Hutchins, C.J. et al., (1986) "Self-cleavage of plus and minus RNA transcripts of avocado sunblotch virus" Nucleic Acids Research, 14: 3627-3635.
Kikuchi, Y. et al., (1991) "Site-specific cleavage of natural mRNA sequences by newly designed hairpin catalytic RNAs" Nucleic Acids Research 19: 6751-6755.
Koizumi, et al., (1988) "Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers" FEBS Letters 228: 228-230.
Koizumi, et al., (1989) "Design of RNA enzymes distinguishing a single base mutation in RNA" Nucleic Acids Research 17: 7059-7071.
Lamb, J.W. and Hay, R.T., (1990) "Ribozymes that cleave potato leafroll virus RNA within the coat protein and polymerase genes", J. Gen. Virol. 71: 2257-2264.
McClain et al., (1987) "Model Substrates for an RNA Enzyme" Science 238: 527-530.
Miller, W.A. et al., (1991) "A Satellite RNA of Barley yellow Dwarf Virus Contains a Novel Hammerhead Structure in the Self-Cleavage Domain" Virology 183: 711-720.
Muesing, M. et al., (1985) "Nucleic Acid Structure and Expression of the Human AIDS/Lymphadenopathy Retrovirus," Nature 313: 450-458.
Prody, G. et al., (1986) "Autolytic Processing of Dimeric Plant Virus Satellite RNA," Science 231: 1577-1580.
Ruffner, D.E. et al., (1989) "Studies on the hammerhead RNA self-cleaving domain"Gene 82: 31-41.
Ruffner, D.E. et al., (1990) "Sequence Requirements of the Hammerhead RNA Self-Cleaving Reaction" Biochemistry 29: 10695-10702.
Sampson et al., (1987) "Characterization of Two-RNA catalyzed RNA Cleavage Reactions" Cold Spring Harbor Sym. Quant. Biol. 52: 267-275.
Sarver, N. et al., (1990) "Ribozymes as Potential Anti-HIV-1 Therapeutic Agents" Science, 247: 1222-1224.
Saxena, S. et al., (1990) "Ribozymes Correctly Cleave a Model Substrate and Endogenous RNA in vivo" J. Biol. Chem. 265: 17106-17109.
Scanlon, K. et al., (1991) "Ribozyme-mediated cleavage of c-fos RNA reduces gene expression of DNA synthesis enzymes and metallothionein" Proc. Natl. Acad. Sci. USA, 88: 10591-10595.
Sheldon, C.C. & Symons, R.H., (1989) "RNA stem stability in the formation of a self-cleaving hammerhead structure" Nucleic Acids Research 17: 5665-5678.
Sheldon, C.C. & Symons, R.H., (1989)"Mutagenesis analysis of a self-cleaving RNA" Nucleic Acids Research, 17: 5679-5686.
Symons, R.H., (1989) "Self-cleavage of RNA in the replication of small pathogens of plants and animals" TIBS 14: 445-450.
Tabler, M. & Tsagris, M., (1991) "Catalytic antisense RNAs produced by incorporating ribozyme cassettes into cDNA" Gene 108: 175-183.
Uhlenbeck et al., (1987) "A small catalytic oligonucleotide" Nature 328: 596-600.
Walbot, V. et al., (1988) "Plant Development and Ribozymes for Pathogens," Nature 334: 196-197.
Waring, R. et al., (1985) "The Tetrahymena rRNA Intron Self-Splices in E. coli: In vivo Evidence for the Importance of key Base-Paired of RNA for RNA Function," Cell 40: 371-380.
Zaug, A.J. & Cech, T.R., (1986A) "The Intervening Sequence RNA of Tetrahymena is an Enzyme" Science 231: 473-474.
Zaug, A.J. et al., (1986B) "The Tetrahymena ribozyme acts like an RNA restriction endonuclease" Nature 234: 429-433.

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