Assay for agents that affect cholesterol 7alpha-hydroxylase expr

Details Assay for agents that affect cholesterol 7alpha-hydroxylase expr Assay for agents that affect cholesterol 7alpha-hydroxylase expr

1995-11-27

1998-10-13

Degen, Nancy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435370, 536 231, C12Q 168, C12N 510, C12N 1511

Patent

active

058210571

ABSTRACT:
DNA regulatory elements that control cholesterol 7.alpha.-hydroxylase (CYP7) expression are disclosed, including a CYP7 minigene that comprises nucleotides downstream from about -371 of the proximal promoter region through about the middle of intron II, inclusive of intron I. This minigene further is stably transfected into a cell line, along with a reporter gene. A method is provided for screening agents that inhibit or stimulate expression of the minigene.

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