Human CCV polypeptides

Details Human CCV polypeptides Human CCV polypeptides

1998-01-21

2003-11-25

Low, Christopher S. F. (Department: 1653)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C530S324000, C530S325000, C530S326000, C530S327000, C530S328000, C435S069100, C435S069700, C435S007100, C435S320100, C536S023100, C536S023500

Reexamination Certificate

active

06653445

ABSTRACT:

FIELD OF THE INVENTION
The present invention relates to genes encoding novel human proteins which exhibit a variety useful biological activities. More specifically, isolated nucleic acid molecules are provided which encode polypeptides comprising various forms of human proteins. Human polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. Also provided are methods for detecting nucleic acids or polypeptides related to those of the invention, for example, to aid in identification of a biological sample or diagnosis of disorders related to expression of protein genes of this invention. The invention further relates to methods for identifying agonists and antagonists of the proteins of the invention, as well as to methods for treatment of disorders related to protein gene expression using polypeptides, antagonists and agonists of the invention.
BACKGROUND OF THE INVENTION
Identification and sequencing of human genes is a major goal of modem scientific research. For example, by identifying genes and determining their sequences, scientists have been able to make large quantities of valuable human gene products. These include human insulin, interferon, Factor VIII, human growth hormone, tissue plasminogen activator, erythropoeitin and numerous other proteins. Additionally, knowledge of gene sequences can provide keys to diagnosis, treatment or cure of genetic diseases such as muscular dystrophy and cystic fibrosis.
Despite the great progress that has been made in recent years, only a small number of genes which encode the presumably thousands of human proteins have been identified and sequenced. Therefore, there is a need for identification and characterization of novel human proteins and corresponding genes which can play a role in detecting, preventing, ameliorating or correcting disorders related to abnormal expression of and responses to such proteins.
SUMMARY OF THE INVENTION
The present invention provides isolated nucleic acid molecules comprising polynucleotide sequences which have been identified as sequences encoding human proteins of the invention. Each protein of the invention is identified in Table 1, below (see Example 2) by a reference number designated as a “Protein ID (Identifier)” (e.g., “PF353-01”). Each protein of the invention is related to a human complementary DNA (cDNA) clone prepared from a messenger RNA (MRNA) encoding the related protein. The cDNA clone related to each protein of the invention is identified by a “cDNA Clone ID (Identifier)” in Table 1 (e.g., “HABCE99”). DNA of each CDNA clone in Table 1 is contained in the material deposited with the American Type Culture Collection and given the ATCC Deposit Number shown for each cDNA Clone ID in Table 1, as further described below.
The invention provides a nucleotide sequence determined for an mRNA molecule encoding each protein identified in Table 1, which is designated in Table 1 as the “Total NT (Nucleotide) Sequence.” This determined nucleotide sequence has been assigned a SEQ ID NO=“X” in the Sequence Listing hereinbelow, where the value of X for the determined nucleotide sequence of each protein is an integer specified in Table 1. The determined nucleotide sequence provided for each protein of the invention was determined by applying conventional automated nucleotide sequencing methods to DNA of the corresponding deposited cDNA clone cited in Table 1.
The determined nucleotide sequence for the mRNA encoding each protein of the invention has been translated to provide a determined amino acid sequence for each protein which is identified in Table 1 by a SEQ ID NO=“Y” where the value of Y for each protein is an integer defined in Table 1. The determined amino acid sequence for each protein represents the amino acid sequence encoded by the determined nucleotide sequence, beginning at or near the translation initiation (“start”) codon of the protein and continuing until the first translation termination (“stop”) codon. Due to possible errors inherent in determining nucleotide sequences from any DNA molecule, particularly using the conventional automated sequencing technology used to sequence the cDNA clones described herein, occasional nucleotide sequence errors are expected in the determined nucleotide sequences of the invention. These errors may include insertions or deletions of one or a few nucleotides in the determined nucleotide sequence as compared to the actual nucleotide sequence of the deposited cDNA. As one of ordinary skill would appreciate, incorrect insertions or deletions of one or two nucleotides into a determined nucleotide sequence leads to a shift in the translation reading frame compared to the reading frame actually encoded by a cDNA clone. Further, such a shift in frame within an actual open reading frame frequently leads to the appearance of a translation termination (stop) codon within the sequence encoding the polypeptide. Accordingly, due to occasional errors in the nucleotide sequences determined from the deposited cDNAs and any related DNA clones used to prepare the determined sequence for the mRNA encoding each secreted protein of the invention, the translations shown as determined amino acid sequences in SEQ ID NO:Y may represent only a portion of the complete amino acid sequence of the human secreted protein actually encoded by the mRNA represented by the corresponding cDNA clone in the ATCC deposit identified in Table 1. In any event, the determined amino acid sequence for each protein in Table 1, which is shown in SEQ ID NO:Y for each protein, comprises at least a portion of the amino acid sequence determined for that protein.
More particularly, the determined amino acid sequence is the amino acid sequence translated from the determined nucleotide sequence in the open reading frame of the first amino acid of the ORF to the last amino acid of that frame. In other words, the determined amino acid sequence is translated from the determined nucleotide sequence beginning at the codon having as its 5′ end the nucleotide in the position of SEQ ID NO:X identified in Table 1 as the 5′ nucleotide of the first amino acid (abbreviated in Table 1 as “5′ NT of First AA”). Translation of the determined nucleotide sequence is continued in the reading frame of that first amino acid codon to the first stop codon in that same open reading frame, i.e., to the position in SEQ ID NO:X which encodes the amino acid at the position in SEQ ID NO:Y identified as the “last amino acid of the open reading frame” (abbreviated as “Last AA of ORF”).
For any determined amino acid sequence in which the first amino acid is the methionine encoded by the translation initiation codon for the protein, Table 1 also identifies the position in SEQ ID NO:X of the 5′ nucleotide of the start codon (“5′ NT of Start Codon”) as the same position in SEQ ID NO:X as that of the 5′ nucleotide of the first amino acid (“First AA”).
Table 1 also identifies the positions in SEQ ID NO:Y of the last amino acid of the signal peptide (“Last AA of Sig Pep”) and the first amino acid of the secreted portion (“First AA of Secreted Portion”) of the protein, for those polypeptide having a secretory leader sequence. The “secreted portion” of a secreted protein in the present context indicates that portion of the complete polypeptide translated from an mRNA which remains after cleavage of the signal peptide by a signal peptidase. In this context the term “mature” may also be used interchangeably with “secreted portion” although it is recognized that in other contexts “mature” may designate a portion of a “proprotein” which is produced by further cleavage of the polypeptide after cleavage of the signal peptide.
Accordingly, in one aspect the invention provides an isolated nucleic acid molecule comprising a nucleotide sequence which is identical to the nucleotide sequence of SEQ ID NO:X, where X is any integer as defined in Table 1. The invention also provides an isolated nucleic acid molecule comprising a nucleotide sequence which is ident

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