Assay using recombinant histidyl-tRNA synthetase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

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435 691, 530350, C12N 900, C12N 500, C12P 2106, C07K 100

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056630662

ABSTRACT:
Recombinant Histidyl tRNA synthetase produced by non-mammalian host cells is used in a sensitive assay to determine the presence of autoimmune diseases in mammals. Methods for isolating, cloning and expressing rHRS are described. In addition, a kit for determining the presence of an autoimmune disease is provided.

REFERENCES:
patent: 5484703 (1996-01-01), Raben et al.
Alberts et al. (1989) Molecular Biology of the Cell, Second Edition, Garland Publishing. NY, NY.
Biswas, et al. "Stimulation and Partial Stabilization of Human Histidyl-tRNA Synthetase by Hemoglobin" FEB 229(1): 203-205 (1988).
Biswas, et al. "An Efficient Method for Enrichment of Histidyl-tRNA Synthetase from HeLa Cells" J. of Immunological Methods 98: 235-241 (1987).
Cusack, et al. "Sequence, Structural and Evolutionary Relationships Between Class 2 Aminoacyl-tRNA Synthetases" Nucl. Acids Res. 19(13): 3489-3498 (1991).
Eriani, et al. "Partition of tRNA Synthetases into Two Classes Based on Mutually Exclusive Sets of Sequence Motifs" Nature 347: 203-206 (1990).
Fett, et al. "The Primary Structure of Human Glutaminyl-tRNA Synthetase" J. of Biol. Chem. 266(3): 1448-1455 (1991).
Garret, et al. "A Mammalian Tryptophanyl-tRNA Synthetase Shows Little Homology to Prokaryotic Synthetases but Near Identity with Mammalian Peptide Chain Release Factor" Biochemistry 30: 7809-7817 (1991).
Love, et al. "A New Approach to the Classification of Idiopathic Inflammatory Myopathy: Myositis-Specific Autoanitbodies Define Useful Homogeneous Patient Groups" Medicine 70(6): 360-373 (1991).
Miller, et al. "The Role of an Autoanitgen, Histidyl-tRNA Synthetase, in The Induction and Maintenance of Autoimmunity" Proc. Natl. Acad. Sci. 87: 9933-9937 (1990).
Jacobo-Molina, et al. "cDNA Sequence, Predicted Primary Structure, and Evolving Amphiphilic Helix of Human Aspartyl-tRNA Synthetase" J. of Biol. Chem. 264(28): 16608-16612 (1989).
Natsoulis, et al. "The HTS1 Gene Encodes Both the Cytoplasmic and Mitochondrial Histidine tRNA Synthetases of S. cerevisiae" Cell 46: 235-243 (1986).
Raben, et al. "Human Histidyl-tRNA Synthetase: Recognition of Amino Acid Signature Regions in Class 2a Aminoacyl-tRNA Synthetases" Nucl. Acids Res. 20(5): 1075-1081 (1992).
Ramsden, et al. "Epitope Mapping of the Cloned Human Autoantigen, Histidyl-tRNA Synthetase" J. of Immunology 143(7): 2267-2272 (1989).
Ruff, et al. "Class II Aminoacyl Transfer RNA Synthetases: Crystal Structure of Yeast Aspartyl-tRNA Synthetase Complexed with tRNA.sup.Asp " Science 252: 1682-1689 (1991).
Targoff, et al. "Measurement of Antibody to Jo-1 by Elisa and Comparison to Enzyme Inhibitory Activity" J. of Immunology 138 (9): 2874-2882 (1987).
Tsui, et al. "Mapping the Epitopes on Jo-1 (Histidyl-tRNA Synthetase)" Arthritis & Rheumatism 31(4 Suppl): B35 (1988).
Tsui, et al. "Amplification of the Gene for Histidyl-tRNA Synthetase in Histidinol-Resistant Chinese Hamster Ovary Cells" Mol. and Cell. Biol. 5(9): 2381-2388 (1985).
Tsui, et al. "Isolation, Structure and Expression of Mammalian Genes for Histidyl-tRNA Synthetase" Nucl. Acids Res. 15(8): 3349-3367 (1987).
Tsui, et al. "Structural Analysis of the 5' Region of the Chromosomal Gene for Hamster Histidyl-tRNA Synthetase" Gene 61: 349-361 (1987).
Eisenbeis, et al. "The Nucleotide Sequence of the Promoter Region of hisS, the Structrual Gene for Histidyl-tRNA Synthetase" Gene 18: 107-114 (1982).
Farhoum, et al. "Purification of Mammalian Histidyl-tRNA Synthetase and Its Interaction with Myositis-Specific Anti Jo-1 Antibodies" Biochemistry 26: 5871-5877 (1987).
Kontis, et al. "Isolation of a cDNA Clone for Human Threonyl-tRNA Synthetase Amplification of the Structural Gene in Borrelidin-Resistant Cell Lines" Mol. and Cell. Biol. 9(5): 1832-1838 (1989).
Raben, et al. "The Correct Sequence of Human Histidyl-tRNA Synthetase, an Important Autoantigen" J. of Cell Biol. (3 Part 2): 89a (1991).
Walker, et al. "Improved Detection of Anti-Jo-1 Antibody, a Marker for Myositis, Using Purified Histidyl-tRNA Synthetase" J. of Immunological Methods 96: 149-156 (1987).
Walker, et al. "Purification of Bovine Liver Histidyl-tRNA Synthetase, the Jo-1 Antigen of Polymyositis" Biol. Chem. Hoppe Seyler 368(5): 531-537 (1987).
Yoshida, et al. "The Precipitating Antibody to an Acidic Nuclear Protein Antigen, the Jo-1, in Connective Tissue Diseases" Arthritis & Rheumatism 26(5): 604-611 (1983).
Freedman, et al. J. Biol. Chem. 260: 10063-10068 (1985).
Nishikai, et al. Arthritis Rheum. 23: 881-888 (1980).

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