Human phospholipase inhibitor

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435325, 4353201, 435348, 435419, 536 235, C12N 1500, C12N 1512, C12N 1563, C12N 1585

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active

056630590

ABSTRACT:
The present invention provides a polynucleotide (gipl) the partial sequence for which was initially isolated from a THP-1 cDNA library and which identifies and encodes a novel human phospholipase inhibitor (GIPL). The invention provides for genetically engineered expression vectors and host cells comprising the nucleic acid sequence encoding GIPL.

REFERENCES:
Ohkura N, et al. "The two subunits of a phospholipase A2 inhibitor from the plasma of thailand cobra having structural similarity to urokinase-type plasminogen activator receptor and ly-6 related proteins." Biochem. Biophys. Res. Commun. 204: 1212-1218. Nov. 15, 1994.
Database EST, Accession No. R16683, Hillier L, et al., Unpublished. Apr. 14, 1995.
de Carvalho et al., "Regulation of Lysophospholipase Activity of the 85kDa Phospholipase A.sub.2 and Activation in Mouse Peritoneal Macrophages", J. Biol. Chem, (1995) 270:20439-20446.
Fortes-Dias et al., "A Phospholipase A.sub.2 Inhibitor from the Plasma of the South American Rattlesnake (Crotalus durissus terrificus)", J. Biol. Chem., (1994) 269:15646-15651.
Gewert et al., "Dexamethasone down-regulates the 85 kDa phospholiphase A.sub.2 in mouse macrophages and suppresses its activation", Biochem. J., (1995), 307:499-504.
Jaattela et al., "Bcl-x and Bcl-2 inhibit TNF and Fas-induced apoptosis and activation of phospholiphase A.sub.2 in breast carcinoma cells", Oncogene, (1995) 10:2297-2305.
Ross et al., "Characterization of a Novel Phospholipase A.sub.2 Activity in Human Brain" J. Neurochem, (1995) 64:2213-2221.
Schalkwijk et al., "Epidermal growth factor (EGF) induces serine phosphorylation-dependent activation and calcium-dependent translocation of the cytosolic phospholipase A.sub.2 " Eur. J. Biochem. (1995) 231:593-601.
Tanaka et al., "Effect of thielocin A1.beta. on bee venom phospholipase A.sub.2 -induced edema in mouse paw" Eur. J. Pharmacol., (1995) 279:143-148.
Trzeciak et al., "Effect of neuroleptics on phospholipase A.sub.2 activity in the brain of rats" Eur. Arch Psychiatry Clin Neurosci., (1995) 245:179-182.
Van den Berg et al., "Solution structure of porcine pancreatic phospholipase A.sub.2 " EMBO J., (1995) 14:4123-4131.
Wijkander et al., "5-Lipoxygenase Products Modulate the Activity of the 85-kDa Phospholipase A.sub.2 in Human Neutrophils" J. Biol. Chem., (1995) 270:26543-26549.
Yamada et al., "Phospholipase A.sub.2 -Activating Peptide-Induced Contraction Of Smooth Muscle Is Mediated By Protein Kinase C-Map Kinase Cascade" Biochem. Biophys Res. Commun., (1995) 217:203-210.

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