Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving fixed or stabilized – nonliving microorganism,...
Patent
1995-06-21
1997-09-02
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving fixed or stabilized, nonliving microorganism,...
435 72, 435 732, 435 733, 435 734, 435 735, 435174, 435176, 4352871, 435968, 436518, 436 10, 436800, G01N 3353, G01N 3300, C12N 1100, C12N 1116
Patent
active
056630573
ABSTRACT:
Process for rapid, ultrasensitive and automatic counting of fluorescent biological cells such as microorganims, carried by a solid support such as a filter.
The process includes: scanning a solid support on which a specimen potentially containing microorganisms has been deposited, with an incident beam from a laser, forming a laser spot on the solid support, the laser spot being substantially greater than the microorganisms to be detected, the laser spot size being between 4 and 14 .mu.m and simultaneously: detecting the resultant fluorescent light at least at one wavelength; establishing a set of correlated-features by a line-to-line correlation of individual features; comparing said correlated-features on each pair of adjacent lines in time synchrony, at least at two different wavelengths .lambda..sub.1 and .lambda..sub.2 ; making a size discrimination of retained events and selecting those having a size corresponding to a microorganism; determining if for retained events after size discrimination, the events energy profile in three dimensions is within predetermined Gaussian shape criteria; and counting said remaining events to determine and to count exclusively the microorganisms present on said solid support.
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Desfetes Patrick
Drocourt Jean-Louis
Sanghera Jaspal
Chemunex
Chin Christopher L.
Nguyen Bao-Thuy L.
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