Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Patent
1994-07-27
1997-09-02
Guzo, David
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
424 936, 4352351, 4353201, A01N 6300, C12N 701
Patent
active
056628978
ABSTRACT:
Insect viruses capable of killing at least one target insect pest quicker than previously described viruses and DNA sequence conferring that phenotype of faster killing are provided. Further improvement in the speed of killing is obtained when the virus of this invention also contains a nonfunctional egt gene to reduce feeding by the infected larvae, inhibit growth and further mediate the earlier death of the infected insect. A specifically exemplified faster-killing insect virus is the V-8 strain of AcMNPV. The faster killing phenotype is carried on a MluI to EspI fragment from 1.93 to 3.27 map units within the AcMNPV genome, and its sequence is provided herein as SEQ ID NO: 3 . . . V8vEGTDEL is the egt-inactivated derivative of AcMNPV V-8; the combination of the increased virulence of the V-8 genotype, for example, and the inactivation of the gene encoding ecdysteroid glycosyl transferase provides further improvement (as further decrease in time after infection until insect death). Additionally, such an Egt-deficient baculovirus may be still further modified to express a protein which affects ecdysis. Methods for producing the faster-killing insect virus, improved insecticidal compositions and improved methods of controlling insects are also included within the scope of this invention.
REFERENCES:
patent: 5180581 (1993-01-01), Miller e tal.
patent: 5246936 (1993-09-01), Treacy et al.
patent: 5266317 (1993-11-01), Tomalski et al.
patent: 5352451 (1994-10-01), Miller et al.
O'Reilly and Miller (1991) "Improvement of a Baculovirus Pesticide by Deletion of the Egt Gene", Biotechnology 9:1086-1089.
Kumar and Miller (1987) "Effects of Serial Passage of Autographa californica Nuclear Polyhedrosis Virus in Cell Culture", Virus Research 7:335-349.
Lee and Miller (1978) "Isolation of Genotypic Variants of Autographa californica Nuclear Polyhedrosis Virus", J. Virol. 27:754-767.
Gearing and Posse (1990) "Functional Analysis of a 603 Nucleotide Open Reading Frame Upstream of the Polyhedrin Gene of Autographa californica Nuclear Polyhedrosis Virus", J. Gen. Virol. 71:251-262.
Passarelli and Miller (1993) "Three Baculovirus Genes Involved in Late and Very Late Gene Expression: ie-l, ie-n, and lef-2", J. Virol. 67:2149-2158.
Vail et al. (1971) "Cross Infectivity of a Nuclear Polyhedrosis Virus isolated from the Alfalfa Looper, Autographa californica", Proc. IV Int. Colloq. Insect Pathology, College Park, MD pp. 297-304.
Possee et al., (1991) "Nucleotide Sequence of the Autographa californica Nuclear Polyhedrosis 9.4 kbp EcoRI-I and -R(Polyhedrin Gene) region", Virology 185:229-241.
Croizier et al. (1988) "Recombination of Autographa californica and Rachiplusia ou Nuclear Polyhedrosis Viruses in Galleria mellonella L.,"J. gen. Virol. 69:177-185.
Possee et al. (1993) "Genetically Engineered Viral Insecticides: New Insecticides with Improved Phenotypes,"Pesticide Science 39:109-115.
Black Bruce Christian
Dierks Peter Michael
Fleming Nancy C.
Miller Lois K.
American Cyanamid Co.
Guzo David
U. of GA Research Foundation
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