Capillary electrophoretic detection of nucleic acids

Details Capillary electrophoretic detection of nucleic acids Capillary electrophoretic detection of nucleic acids

1995-06-06

1999-02-23

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

422 50, 422 62, 422 681, 422 69, 422 8205, 4352861, 4352871, 4352872, 4352891, 436501, 530350, 536 253, 536 254, 935 77, 935 78, 935 88, C12Q 168

Patent

active

058742131

ABSTRACT:
Methods are provided for detection and quantitation of mixtures containing target nucleobase sequences using capillary electrophoresis. Peptide nucleic acid oligomers, complementary to the target sequences and preferably having appended detectable labels, are hybridized to the targets. Capillary electrophoresis is then performed, and the detectable label is detected and quantitated.

REFERENCES:
Chen et al., "Identification on DNA Molecules by Pre-Column Hybridization Using Capillary Electrophoresis", J. of Chromatography 559: 295-305 (1991).
Goodchild, "Conjugates of Oligonucleotides and Modified Oligonucleotides: A Review of Their Synthesis and Properties", Bioconjuage Chemistry vol. 1: 165-187 (1990).
Huang et al., "Bias in Quantitative Capillary Zone Electrophoresis Caused by Electrokinetic Sample Injection", Anal. Chem. 60: 375-377 (1988).
Luckey et al., "High Speed DNA Sequencing By Capillary Electrophoresis", Nucleic Acids Research 18: 4417-4421 (1990).
McCord et al., "Capillary Electrophoresis of Polymerase Chain Reaction-Amplified DNA Using Fluorescence Detection with an Intercalating Dye", J. of Chromatography A 652: 75-82 (1993).
Rose, "Characterization of Antisense Binding Properties of Peptide Nucleic Acids by Capillary Gel Electrophoresis", Anal. Chem. 65: 3545-3549 (1993).
Janson & Ryden, Eds., Protein Purification: Principles High Resolution Methods and Applications., Ch. 17 "Capillary Electrophoretic Separations" (Thormann and Firestone), VCH Publishers, New York, New York (1989).
Matthews et al. (1988) Analytical Biochemistry, vol. 169, pp. 1-25.

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