Method for specifically altering the nucleotide sequence of RNA

Details Method for specifically altering the nucleotide sequence of RNA Method for specifically altering the nucleotide sequence of RNA

1992-08-28

1995-02-14

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 29, 435 911, 435 913, 435 9132, 435 915, 435 9151, C12Q 169, C12Q 102, C12P 1934

Patent

active

053895142

ABSTRACT:
A method is provided for specifically altering the nucleotide sequence of an RNA molecule. As performed in vitro the method comprises: (1) selecting a target sequence in an RNA molecule; (3) hybridizing the target sequence with a complementary nucleic acid, either DNA or RNA, thereby forming a double-stranded structure; (4) incubating the hybrid molecule with a cellular extract which contains components capable of specifically converting U to C in an RNA molecule, when the U is disposed within a double-stranded structure. Incubation of the hybrid molecule with cellular extracts comprising the RNA editing component results in a specific alteration of the nucleotide sequence of the RNA at the target sequence. The method of the invention may also be applied in vivo. Complementary nucleic acids are introduced into cells and hybridized to the target sequence to form a double-stranded structure. The RNA editing components already present in the cell may then specifically convert U to C in the target sequence resulting in an alteration in the target RNA molecule.

REFERENCES:
Casey et al. P.N.A.S. 89: 7149-7153, 1992.
Araya et al. P.N.A.S. 89: 1040-1044, 1992.
H. Zheng et al., Journal of Virology, 66: 4693-4697 (1992),
R. Heiermann et al., Nucleic Acids Research, 13: 2709-2730 (1985).
J. Manley, Proc. Natl. Acad. Sci. USA, 77: 3855-3859 (1980),
J. Glenn et al., Journal of Virology, 64: 3104-3107 (1900).
J. Haseloff et al., Nature, 334: 585-591 (1988).
F. Cameron et al., Proc. Natl. Acad.Sci, USA, 86: 9139-9143 (1989).
R. Wagner et al., Proc. Natl. Acad. Sci., USA, 86: 2647-2651 (1989).
C. Sureau et al., Journal of Virology, 63: 4292-4297 (1989).
G. Luo et al., Journal of Virology, 64: 1021-1027 (1990).
M. Chao et al., Journal of Virology, 64: 5066-5069 (1990).
M. Chao et al., The Hepatitis Delta Virus, Wiley-Liss, Inc., pp. 275-281 (1991).
J. Taylor et al., Journal of Hepatology, 13: S119-S120 (1991).
R. Cattaneo, Experientia, 46: 1142-1148 (1990).
V. Walbot, Trends in Genetics, 7 (1991).
P. Hodges et al., Trends in Biochemical Sciences, 17 (1992).
R. Hurlbert et al., the Journal of Biological Chemistry, 235: 443-449 (1960).
N. Salzman et al., Journal of Biological Chemistry, 230: 1001-1012 (1958).

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