Assay for equine infectious anemia virus

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 79, 435 793, 435 795, 436518, G01N 33543, G01N 33569

Patent

active

054279070

ABSTRACT:
The present invention is directed toward the use of a synthetic peptide as the antigen in an immunoassay for the detection of antibodies against the equine infectious anemia virus in the serum of horses. The synthetic peptide corresponds to the amino acid sequence of an antigenic portion of the GP-45 envelope protein of the equine infectious anemia virus. The immunoassay may be a direct second antibody immunoassay, a one or two step sandwich immunoassay, or a competitive immunoassay.

REFERENCES:
patent: 4806467 (1989-02-01), Porter et al.
Romerser (1983) Equus 116:18-20.
O'Rourke et al (1988) Antuiral Antiglycoproten and Neutralization . . . J Gen Viral 69:667-674.
Ball et al (1988) Lentivirus Antigen purification and characterization . . . J V. rol Meth. 19:265-277.
Rushlow et al (1986) Lentivirus Genomei Organization . . . Virol. 155:309-321.
Hussain et al (1987) Antigenic Analysis of EIAU . . . J Virol 61;2956-2961.
Shoemann et al (1987) Comparison of Recombmant . . . Anal. Biochem 161:370-379.
Clark (1980) The Experimental Foundation of Modern Immunology, J W & Sons P. 5-6.

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